Bruton's tyrosine kinase (Btk): function, regulation, and transformation with special emphasis on the PH domain.

Forskningsoutput: TidskriftsbidragÖversiktsartikel

Abstract

Bruton's agammaglobulinemia tyrosine kinase (Btk) is a cytoplasmic tyrosine kinase important in B-lymphocyte development, differentiation, and signaling. Btk is a member of the Tec family of kinases. Mutations in the Btk gene lead to X-linked agammaglobulinemia (XLA) in humans and X-linked immunodeficiency (Xid) in mice. Activation of Btk triggers a cascade of signaling events that culminates in the generation of calcium mobilization and fluxes, cytoskeletal rearrangements, and transcriptional regulation involving nuclear factor-kappaB (NF-kappaB) and nuclear factor of activated T cells (NFAT). In B cells, NF-kappaB was shown to bind to the Btk promoter and induce transcription, whereas the B-cell receptor-dependent NF-kappaB signaling pathway requires functional Btk. Moreover, Btk activation is tightly regulated by a plethora of other signaling proteins including protein kinase C (PKC), Sab/SH3BP5, and caveolin-1. For example, the prolyl isomerase Pin1 negatively regulates Btk by decreasing tyrosine phosphorylation and steady state levels of Btk. It is intriguing that PKC and Pin1, both of which are negative regulators, bind to the pleckstrin homology domain of Btk. To this end, we describe here novel mutations in the pleckstrin homology domain investigated for their transforming capacity. In particular, we show that the mutant D43R behaves similar to E41K, already known to possess such activity.

Detaljer

Författare
  • Abdalla J Mohamed
  • Liang Yu
  • Carl-Magnus Bäckesjö
  • Leonardo Vargas
  • Rani Faryal
  • Alar Aints
  • Birger Christensson
  • Anna Berglöf
  • Mauno Vihinen
  • Beston F Nore
  • C I Edvard Smith
Externa organisationer
  • External Organization - Unknown
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Immunologi inom det medicinska området

Nyckelord

Originalspråkengelska
Sidor (från-till)58-73
TidskriftImmunological Reviews
Volym228
Utgivningsnummer1
StatusPublished - 2009
PublikationskategoriForskning
Peer review utfördJa
Externt publiceradJa