Calorimetric analysis of cephalosporins using an immobilized TEM-1 beta-lactamase on Ni2+ chelating sepharose fast flow

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

Two beta-lactamases, penicillinase type I from Bacillus cereus and TEM-1 beta-lactamase from Haemophilus ducreyi, were immobilized on a Chelating Sepharose Fast Flow column loaded with Ni2+ in an active form. Flow-injection analysis of beta-lactams was performed by using an enzyme column reactor fitted into the enzyme thermistor. With both enzymes it was possible to monitor both penicillins and cephalosporins. Moreover, Michaelis constants of the TEM-1 beta-lactamase were markedly increased upon immobilization for all substrates, especially carbenicillin, cephaloridine, and cefoperazone.

Detaljer

Författare
  • R Lawung
  • Bengt Danielsson
  • V Prachayasittikul
  • L Bülow
Enheter & grupper
Externa organisationer
  • Mahidol University
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Analytisk kemi
  • Biokemi och molekylärbiologi

Nyckelord

Originalspråkengelska
Sidor (från-till)57-62
Antal sidor6
TidskriftAnalytical Biochemistry
Volym296
Utgåva nummer1
StatusPublished - 2001 sep 1
PublikationskategoriForskning
Peer review utfördJa