Cannula Implantation into the Cisterna Magna of Rodents

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Standard

Cannula Implantation into the Cisterna Magna of Rodents. / Xavier, Anna L.R.; Hauglund, Natalie Linea; von Holstein-Rathlou, Stephanie; Li, Qianliang; Sanggaard, Simon; Lou, Nanhong; Lundgaard, Iben; Nedergaard, Maiken.

I: Journal of visualized experiments : JoVE, Nr. 135, e57378, 23.05.2018.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Harvard

Xavier, ALR, Hauglund, NL, von Holstein-Rathlou, S, Li, Q, Sanggaard, S, Lou, N, Lundgaard, I & Nedergaard, M 2018, 'Cannula Implantation into the Cisterna Magna of Rodents', Journal of visualized experiments : JoVE, nr. 135, e57378. https://doi.org/10.3791/57378

APA

Xavier, A. L. R., Hauglund, N. L., von Holstein-Rathlou, S., Li, Q., Sanggaard, S., Lou, N., Lundgaard, I., & Nedergaard, M. (2018). Cannula Implantation into the Cisterna Magna of Rodents. Journal of visualized experiments : JoVE, (135), [e57378]. https://doi.org/10.3791/57378

CBE

Xavier ALR, Hauglund NL, von Holstein-Rathlou S, Li Q, Sanggaard S, Lou N, Lundgaard I, Nedergaard M. 2018. Cannula Implantation into the Cisterna Magna of Rodents. Journal of visualized experiments : JoVE. (135):Article e57378. https://doi.org/10.3791/57378

MLA

Vancouver

Xavier ALR, Hauglund NL, von Holstein-Rathlou S, Li Q, Sanggaard S, Lou N et al. Cannula Implantation into the Cisterna Magna of Rodents. Journal of visualized experiments : JoVE. 2018 maj 23;(135). e57378. https://doi.org/10.3791/57378

Author

Xavier, Anna L.R. ; Hauglund, Natalie Linea ; von Holstein-Rathlou, Stephanie ; Li, Qianliang ; Sanggaard, Simon ; Lou, Nanhong ; Lundgaard, Iben ; Nedergaard, Maiken. / Cannula Implantation into the Cisterna Magna of Rodents. I: Journal of visualized experiments : JoVE. 2018 ; Nr. 135.

RIS

TY - JOUR

T1 - Cannula Implantation into the Cisterna Magna of Rodents

AU - Xavier, Anna L.R.

AU - Hauglund, Natalie Linea

AU - von Holstein-Rathlou, Stephanie

AU - Li, Qianliang

AU - Sanggaard, Simon

AU - Lou, Nanhong

AU - Lundgaard, Iben

AU - Nedergaard, Maiken

PY - 2018/5/23

Y1 - 2018/5/23

N2 - Cisterna magna cannulation (CMc) is a straightforward procedure that enables direct access to the cerebrospinal fluid (CSF) without operative damage to the skull or the brain parenchyma. In anesthetized rodents, the exposure of the dura mater by blunt dissection of the neck muscles allows the insertion of a cannula into the cisterna magna (CM). The cannula, composed either by a fine beveled needle or borosilicate capillary, is attached via a polyethylene (PE) tube to a syringe. Using a syringe pump, molecules can then be injected at controlled rates directly into the CM, which is continuous with the subarachnoid space. From the subarachnoid space, we can trace CSF fluxes by convective flow into the perivascular space around penetrating arterioles, where solute exchange with the interstitial fluid (ISF) occurs. CMc can be performed for acute injections immediately following the surgery, or for chronic implantation, with later injection in anesthetized or awake, freely moving rodents. Quantitation of tracer distribution in the brain parenchyma can be performed by epifluorescence, 2-photon microscopy, and magnetic resonance imaging (MRI), depending on the physico-chemical properties of the injected molecules. Thus, CMc in conjunction with various imaging techniques offers a powerful tool for assessment of the glymphatic system and CSF dynamics and function. Furthermore, CMc can be utilized as a conduit for fast, brain-wide delivery of signaling molecules and metabolic substrates that could not otherwise cross the blood brain barrier (BBB).

AB - Cisterna magna cannulation (CMc) is a straightforward procedure that enables direct access to the cerebrospinal fluid (CSF) without operative damage to the skull or the brain parenchyma. In anesthetized rodents, the exposure of the dura mater by blunt dissection of the neck muscles allows the insertion of a cannula into the cisterna magna (CM). The cannula, composed either by a fine beveled needle or borosilicate capillary, is attached via a polyethylene (PE) tube to a syringe. Using a syringe pump, molecules can then be injected at controlled rates directly into the CM, which is continuous with the subarachnoid space. From the subarachnoid space, we can trace CSF fluxes by convective flow into the perivascular space around penetrating arterioles, where solute exchange with the interstitial fluid (ISF) occurs. CMc can be performed for acute injections immediately following the surgery, or for chronic implantation, with later injection in anesthetized or awake, freely moving rodents. Quantitation of tracer distribution in the brain parenchyma can be performed by epifluorescence, 2-photon microscopy, and magnetic resonance imaging (MRI), depending on the physico-chemical properties of the injected molecules. Thus, CMc in conjunction with various imaging techniques offers a powerful tool for assessment of the glymphatic system and CSF dynamics and function. Furthermore, CMc can be utilized as a conduit for fast, brain-wide delivery of signaling molecules and metabolic substrates that could not otherwise cross the blood brain barrier (BBB).

U2 - 10.3791/57378

DO - 10.3791/57378

M3 - Article

C2 - 29889209

AN - SCOPUS:85051988680

JO - Journal of Visualized Experiments

JF - Journal of Visualized Experiments

SN - 1940-087X

IS - 135

M1 - e57378

ER -