Capsaicin receptor immunoreactivity in the human trigeminal ganglion.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Standard

Capsaicin receptor immunoreactivity in the human trigeminal ganglion. / Hou, Mingyan; Uddman, Rolf; Tajti, Janos; Kanje, Martin; Edvinsson, Lars.

I: Neuroscience Letters, Vol. 330, Nr. 3, 2002, s. 223-226.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Harvard

APA

CBE

MLA

Vancouver

Author

Hou, Mingyan ; Uddman, Rolf ; Tajti, Janos ; Kanje, Martin ; Edvinsson, Lars. / Capsaicin receptor immunoreactivity in the human trigeminal ganglion. I: Neuroscience Letters. 2002 ; Vol. 330, Nr. 3. s. 223-226.

RIS

TY - JOUR

T1 - Capsaicin receptor immunoreactivity in the human trigeminal ganglion.

AU - Hou, Mingyan

AU - Uddman, Rolf

AU - Tajti, Janos

AU - Kanje, Martin

AU - Edvinsson, Lars

PY - 2002

Y1 - 2002

N2 - The cloned capsaicin receptor, also known as vanilloid receptor subtype 1 (VR1) receptor, has been demonstrated to be an integral membrane protein with homology to a family of putative store-operated calcium channels. The VR1 receptor is activated not only by capsaicin but also by noxious heat and protons, and therefore it is suggested as a molecular integrator of chemical and physical stimuli that elicit pain. In the present study, indirect immunofluorescence detected a small number of neurons that are VR1 receptor immunoreactive (ir) (171 versus 1038 or 16% of all neuronal cell bodies) in the human trigeminal ganglion (TG). In addition, RT-PCR confirmed the presence of VR1 mRNA in the human TG. It has been hypothesized that TG neuronal cell bodies are the source of capsaicin-stimulated release of calcitonin gene-related peptide (CGRP), and hence co-localization experiments were performed. Around 10% of the VR1 receptor-ir is expressed on neurons that contain CGRP-ir (ten among 74) in the human TG, indicating that capsaicin may act through the VR1 receptor to modulate the release of CGRP and in turn to modulate pain. We observed that 8% of the VR1 receptor-ir neuronal cell bodies contain substance P-ir and 5% nitric oxide synthase. Capsaicin can release nitric oxide, CGRP and substance P from sensory nerves and contribute to central sensitization.

AB - The cloned capsaicin receptor, also known as vanilloid receptor subtype 1 (VR1) receptor, has been demonstrated to be an integral membrane protein with homology to a family of putative store-operated calcium channels. The VR1 receptor is activated not only by capsaicin but also by noxious heat and protons, and therefore it is suggested as a molecular integrator of chemical and physical stimuli that elicit pain. In the present study, indirect immunofluorescence detected a small number of neurons that are VR1 receptor immunoreactive (ir) (171 versus 1038 or 16% of all neuronal cell bodies) in the human trigeminal ganglion (TG). In addition, RT-PCR confirmed the presence of VR1 mRNA in the human TG. It has been hypothesized that TG neuronal cell bodies are the source of capsaicin-stimulated release of calcitonin gene-related peptide (CGRP), and hence co-localization experiments were performed. Around 10% of the VR1 receptor-ir is expressed on neurons that contain CGRP-ir (ten among 74) in the human TG, indicating that capsaicin may act through the VR1 receptor to modulate the release of CGRP and in turn to modulate pain. We observed that 8% of the VR1 receptor-ir neuronal cell bodies contain substance P-ir and 5% nitric oxide synthase. Capsaicin can release nitric oxide, CGRP and substance P from sensory nerves and contribute to central sensitization.

U2 - 10.1016/S0304-3940(02)00741-3

DO - 10.1016/S0304-3940(02)00741-3

M3 - Article

VL - 330

SP - 223

EP - 226

JO - Neuroscience Letters

JF - Neuroscience Letters

SN - 0304-3940

IS - 3

ER -