Carbon ceramic electrodes modified with laccase from Trametes hirsuta: Fabrication, characterization and their use for phenolic compounds detection

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Abstract

Fungal laccase (Lc) from the basidiomycete Trametes hirsuta was immobilized on top of a carbon ceramic electrode using physical absorption. Direct, unmediated heterogeneous electron transfer between Lc and the carbon ceramic electrode (CCE) under aerobic conditions was shown. The bioelectrocatalytic reduction of oxygen on Lc-CCE started at about 430 mV vs. Ag vertical bar AgCl vertical bar KClsat at pH 3.5 and moved with about 57 mV in the cathodic region per pH unit. The Lc-modified CCE was then used as a biosensing detection element in a single line flow injection system for the amperometric determination of a variety of phenolic substrates of the enzyme. The experimental conditions were studied and optimized for catechol serving as a model compound. Statistical aspects were applied and the sensor characteristics and Michaelis-Menten constants of the investigated phenolic compounds were calculated and compared with those obtained for solid graphite electrodes modified with Trametes hirsuta laccase. The results showed that the CCE based biosensor in comparison with the solid graphite based biosensor offers a lower detection limit, a wider linear dynamic range, and excellent operational stability with no sensor passivation, indicating that the sol - gel lattice improves the electrochemical behavior of the biosensor.

Detaljer

Författare
  • Behzad Haghighi
  • Amir Rahmati-Panah
  • Sergey Shleev
  • Lo Gorton
Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Analytisk kemi

Nyckelord

Originalspråkengelska
Sidor (från-till)907-917
TidskriftElectroanalysis
Volym19
Utgåva nummer9
StatusPublished - 2007
PublikationskategoriForskning
Peer review utfördJa