CD14(hi) HLA-DRdim macrophages, with a resemblance to classical blood monocytes, dominate inflamed mucosa in Crohn's disease

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift


The increase of CD14(+) lamina propria macrophages in active Crohn's disease is due to a subset discriminated by HLA-DR, likely from CD16(-) blood monocytes. Intestinal M phi play an important role in maintaining gut homeostasis. However, little is known about these cells, their precursors, and their role in intestinal inflammation. Here, we characterize the CD14(+) mononuclear cell populations in intestinal mucosa and blood in patients with CD. Among the LP CD14(+) M phi, we identified three distinct HLA-DR+-expressing subsets. Compared with uninflamed, inflamed mucosa contained a marked increase in the proportion of the CD14(hi)HLA-DRdim cellular subset. This subset resembled the classical blood monocytes with low CD16, HLA-DR, and CX3CR1 expression. Classical monocytes migrated efficiently toward CCL2 and released the highest levels of MMP-1 and proinflammatory cytokines when stimulated with immune complexes or LPS. Our findings strongly suggest that it is the classical and not the intermediate or nonclassical monocytes that are the precursors to the dominating intestinal CD14(hi)HLA-DRdim subset. This enhances our understanding of CD pathology and may provide new options in treatment.


  • Susanne Thiesen
  • Sabina Janciauskiene
  • Heli Uronen-Hansson
  • William Agace
  • Carl-Magnus Hogerkorp
  • Pieter Spee
  • Katarina Hakansson
  • Olof Grip
Enheter & grupper

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Cell- och molekylärbiologi


Sidor (från-till)531-541
TidskriftJournal of Leukocyte Biology
StatusPublished - 2014
Peer review utfördJa

Relaterad forskningsoutput

Thiesen, S., 2015, Gastroenterology, Dept of Clinical Sciences, Malmö, Lund University. 82 s.

Forskningsoutput: AvhandlingDoktorsavhandling (sammanläggning)

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