Cell and tissue manipulation with ultrashort infrared laser pulses in light-sheet microscopy

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

Three-dimensional live imaging has become an indispensable technique in the fields of cell, developmental and neural biology. Precise spatio-temporal manipulation of biological entities is often required for a deeper functional understanding of the underlying biological process. Here we present a home-built integrated framework and optical design that combines three-dimensional light-sheet imaging over time with precise spatio-temporal optical manipulations induced by short infrared laser pulses. We demonstrate their potential for sub-cellular ablation of neurons and nuclei, tissue cauterization and optogenetics by using the Drosophila melanogaster and zebrafish model systems.

Detaljer

Författare
  • Gustavo de Medeiros
  • Dimitri Kromm
  • Balint Balazs
  • Nils Norlin
  • Stefan Günther
  • Emiliano Izquierdo
  • Paolo Ronchi
  • Shinya Komoto
  • Uros Krzic
  • Yannick Schwab
  • Francesca Peri
  • Stefano de Renzis
  • Maria Leptin
  • Matteo Rauzi
  • Lars Hufnagel
Externa organisationer
  • European Molecular Biology Laboratory Heidelberg
  • Friedrich Miescher Institute for Biomedical Research
  • University of Zurich
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Cell- och molekylärbiologi
Originalspråkengelska
Sidor (från-till)1942
TidskriftScientific Reports
Volym10
Utgåva nummer1
StatusPublished - 2020 feb 6
PublikationskategoriForskning
Peer review utfördJa
Externt publiceradJa