Cell immobilization on 3D-printed matrices: A model study on propionic acid fermentation

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Cell immobilization on 3D-printed matrices : A model study on propionic acid fermentation. / Belgrano, Fabricio dos Santos; Diegel, Olaf; Pereira, Nei; Hatti-Kaul, Rajni.

I: Bioresource Technology, Vol. 249, 01.02.2018, s. 777-782.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

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TY - JOUR

T1 - Cell immobilization on 3D-printed matrices

T2 - A model study on propionic acid fermentation

AU - Belgrano, Fabricio dos Santos

AU - Diegel, Olaf

AU - Pereira, Nei

AU - Hatti-Kaul, Rajni

PY - 2018/2/1

Y1 - 2018/2/1

N2 - This study uses three-dimensional (3D) printing technology as a tool for designing carriers for immobilization of microbial cells for bioprocesses. Production of propionic acid from glucose by immobilized Propionibacterium sp. cells was studied as a model system. For cell adsorption, the 3D-printed nylon beads were added to the culture medium during 3 rounds of cell cultivation. Cell adsorption and fermentation kinetics were similar irrespective of the bead size and lattice structure. The cells bound to 15 mm beads exhibited reduced fermentation time as compared to free cell fermentations; maximum productivity and propionic acid titer of 0.46 g/L h and 25.8 g/L, respectively, were obtained. Treatment of the beads with polyethyleneimine improved cell-matrix binding, but lowered the productivity perhaps due to inhibitory effect of the polycation. Scanning electron micrographs revealed the cells to be located in crevices of the beads, but were more uniformly distributed on PEI-coated carrier indicating charge-charge interaction.

AB - This study uses three-dimensional (3D) printing technology as a tool for designing carriers for immobilization of microbial cells for bioprocesses. Production of propionic acid from glucose by immobilized Propionibacterium sp. cells was studied as a model system. For cell adsorption, the 3D-printed nylon beads were added to the culture medium during 3 rounds of cell cultivation. Cell adsorption and fermentation kinetics were similar irrespective of the bead size and lattice structure. The cells bound to 15 mm beads exhibited reduced fermentation time as compared to free cell fermentations; maximum productivity and propionic acid titer of 0.46 g/L h and 25.8 g/L, respectively, were obtained. Treatment of the beads with polyethyleneimine improved cell-matrix binding, but lowered the productivity perhaps due to inhibitory effect of the polycation. Scanning electron micrographs revealed the cells to be located in crevices of the beads, but were more uniformly distributed on PEI-coated carrier indicating charge-charge interaction.

KW - 3D-printed matrix

KW - Cell immobilization

KW - High cell density fermentation

KW - Propionic acid fermentation

U2 - 10.1016/j.biortech.2017.10.087

DO - 10.1016/j.biortech.2017.10.087

M3 - Article

VL - 249

SP - 777

EP - 782

JO - Bioresource Technology

JF - Bioresource Technology

SN - 0960-8524

ER -