Cellular traction forces: a useful parameter in cancer research

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

The search for new cancer biomarkers is essential for fundamental research, diagnostics, as well as for patient treatment and monitoring. Whereas most cancer biomarkers are biomolecules, an increasing number of studies show that mechanical cues are promising biomarker candidates. Although cell deformability has been shown to be a possible cancer biomarker, cellular forces as cancer biomarkers have been left largely unexplored. Here, we measure traction forces of cancer and normal-like cells at high spatial resolution using a robust method based on dense vertical arrays of nanowires. A force map is created using automated image analysis based on the localization of the fluorescent tips of the nanowires. We show that the force distribution and magnitude differ between MCF7 breast cancer cells and MCF10A normal-like breast epithelial cells, and that monitoring traction forces can be used to investigate the effects of anticancer drugs.

Detaljer

Författare
Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Cancer och onkologi
  • Radiologi och bildbehandling

Nyckelord

Originalspråkengelska
Sidor (från-till)19039-19044
Antal sidor6
TidskriftNanoscale
Volym9
Utgivningsnummer48
StatusPublished - 2017 dec 14
PublikationskategoriForskning
Peer review utfördJa