Cellular traction forces: a useful parameter in cancer research

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Cellular traction forces : a useful parameter in cancer research. / Li, Zhen; Persson, Henrik; Adolfsson, Karl; Abariute, Laura; Borgström, Magnus T; Hessman, Dan; Åström, Kalle; Oredsson, Stina; Prinz, Christelle N.

I: Nanoscale, Vol. 9, Nr. 48, 14.12.2017, s. 19039-19044.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

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TY - JOUR

T1 - Cellular traction forces

T2 - Nanoscale

AU - Li, Zhen

AU - Persson, Henrik

AU - Adolfsson, Karl

AU - Abariute, Laura

AU - Borgström, Magnus T

AU - Hessman, Dan

AU - Åström, Kalle

AU - Oredsson, Stina

AU - Prinz, Christelle N

PY - 2017/12/14

Y1 - 2017/12/14

N2 - The search for new cancer biomarkers is essential for fundamental research, diagnostics, as well as for patient treatment and monitoring. Whereas most cancer biomarkers are biomolecules, an increasing number of studies show that mechanical cues are promising biomarker candidates. Although cell deformability has been shown to be a possible cancer biomarker, cellular forces as cancer biomarkers have been left largely unexplored. Here, we measure traction forces of cancer and normal-like cells at high spatial resolution using a robust method based on dense vertical arrays of nanowires. A force map is created using automated image analysis based on the localization of the fluorescent tips of the nanowires. We show that the force distribution and magnitude differ between MCF7 breast cancer cells and MCF10A normal-like breast epithelial cells, and that monitoring traction forces can be used to investigate the effects of anticancer drugs.

AB - The search for new cancer biomarkers is essential for fundamental research, diagnostics, as well as for patient treatment and monitoring. Whereas most cancer biomarkers are biomolecules, an increasing number of studies show that mechanical cues are promising biomarker candidates. Although cell deformability has been shown to be a possible cancer biomarker, cellular forces as cancer biomarkers have been left largely unexplored. Here, we measure traction forces of cancer and normal-like cells at high spatial resolution using a robust method based on dense vertical arrays of nanowires. A force map is created using automated image analysis based on the localization of the fluorescent tips of the nanowires. We show that the force distribution and magnitude differ between MCF7 breast cancer cells and MCF10A normal-like breast epithelial cells, and that monitoring traction forces can be used to investigate the effects of anticancer drugs.

KW - Journal Article

U2 - 10.1039/c7nr06284b

DO - 10.1039/c7nr06284b

M3 - Article

VL - 9

SP - 19039

EP - 19044

JO - Nanoscale

JF - Nanoscale

SN - 2040-3372

IS - 48

ER -