Cellular Uptake of Cystatin C. Subcellular localisation and intracellular effects of a secreted cysteine protease inhibitor

Forskningsoutput: AvhandlingDoktorsavhandling (sammanläggning)

Abstract

Popular Abstract in English
The secreted cysteine protease inhibitor cystatin C has been thought to exert its functions extracellularly, as a defense against enzymes from leaking lysosomes or invading pathogens. In this thesis we demonstrate that regulation of intracellular proteases by cystatin C is possible, as a result of cellular uptake, and that the uptake can be modulated by molecular engineering of wild-type cystatin C. The uptake was seen in different epithelial and neuroblastoma cancer cell lines and was shown by various techniques, including flow cytometry, confocal microscopy, ELISA and Western blotting. Intracellular cysteine proteases involved in cancer-promoting processes may thus be controlled by cystatin C uptake.

Detaljer

Författare
Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Farmakologi och toxikologi
  • Läkemedelskemi

Nyckelord

Originalspråkengelska
KvalifikationDoktor
Tilldelande institution
Handledare/Biträdande handledare
Tilldelningsdatum2013 sep 13
Förlag
  • Division of Clinical Chemistry and Pharmacology, Faculty of Medicine, Lund University
Tryckta ISBN978-91-87449-57-4
StatusPublished - 2013
PublikationskategoriForskning

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