Cellulase production by T-reesei

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Abstract

Steam-pretreated willow has been found to be a suitable substrate for ethanol production in Sweden. The production of Trichoderma reesei cellulases on steam-pretreated willow (SPW) of varying cellulose content has been studied. Batch fermentations were performed in shake flasks, and in 4- and 22-l fermenters. SPW was found to be a suitable carbon source for cellulase production. At an initial solids concentration corresponding to 10 g/l cellulose, the yield was 108 filter paper units (FPU)/g cellulose after 3 days in the 22-l fermenter However, the yield was below 100 FPU/g cellulose in the shake flasks and 4-l fermenter The solid residue of enzymatically hydrolysed SPW was found to be nearly as good a substrate as SPW, although the cellulose content was as low as 20% in this hydrolysis residue. Using delignified SPW, the yield of cellulases was low, indicating that lignin does not inhibit cellulase production. By applying a pressure of 1.7 bar in the fermenter, the initial fermentation rate was increased. The productivity was increased from 12.2 FPU/l/h to 16.7 FPU/l/h, due to the higher level of dissolved oxygen at the higher pressure. Copyright (C) 1996 Elsevier Science Ltd.

Detaljer

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Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Kemiteknik

Nyckelord

Originalspråkengelska
Sidor (från-till)25-30
TidskriftBioresource Technology
Volym57
Utgivningsnummer1
StatusPublished - 1996
PublikationskategoriForskning
Peer review utfördJa