Characterization of a novel testicular form of human hormone-sensitive lipase

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

Hormone-sensitive lipase (HSL) is an esterase and lipase, which are essential for spermatogenesis. Two HSL mRNAs are expressed in human testis. A long form is encoded by a testis-specific exon and nine exons common to testis and adipocyte HSL. Here we show that the short-form 3.3-kb mRNA possesses a unique 5' end that is transcribed from a novel testis-specific exon. The corresponding protein is similar to the 775-amino-acid-long adipocyte HSL. Immunohistochemistry experiments on human testis sections revealed that the long form is strictly expressed in haploid germ cells whereas the short form is expressed in interstitial and tubular somatic cells as well as premeiotic germ cells.

Detaljer

Författare
  • A Mairal
  • N Melaine
  • H Laurell
  • J Grober
  • Lena Stenson
  • T Guillaudeux
  • Cecilia Holm
  • B Jegou
  • D Langin
Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Biologiska vetenskaper

Nyckelord

Originalspråkengelska
Sidor (från-till)286-290
TidskriftBiochemical and Biophysical Research Communications
Volym291
Utgivningsnummer2
StatusPublished - 2002
PublikationskategoriForskning
Peer review utfördJa