Characterization of the biochemical and biophysical properties of the Saccharomyces cerevisiae phosphate transporter Pho89

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

In Saccharomyces cerevisiae, Pho89 mediates a cation-dependent transport of Pi across the plasma membrane. This integral membrane protein belongs to the Inorganic Phosphate Transporter (PiT) family, a group that includes the mammalian Na(+)/Pi cotransporters Pit1 and Pit2. Here we report that the Pichia pastoris expressed recombinant Pho89 was purified in the presence of Foscholine-12 and functionally reconstituted into proteoliposomes with a similar substrate specificity as observed in an intact cell system. The alpha-helical content of the Pho89 protein was estimated to 44%. EPR analysis showed that purified Pho89 protein undergoes conformational change upon addition of substrate.

Detaljer

Författare
Enheter & grupper
Externa organisationer
  • Linnaeus University
  • University of California, Davis
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Biologiska vetenskaper

Nyckelord

Originalspråkengelska
Sidor (från-till)551-556
Antal sidor6
TidskriftBiochemical and Biophysical Research Communications
Volym436
Utgivningsnummer3
StatusPublished - 2013 jul 5
PublikationskategoriForskning
Peer review utfördJa