Chronic exposure to cadmium and arsenic strongly influences concentrations of 8-oxo-7,8-dihydro-2'-deoxyguanosine in urine.

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Chronic exposure to cadmium and arsenic strongly influences concentrations of 8-oxo-7,8-dihydro-2'-deoxyguanosine in urine. / Engström, Karin; Vahter, Marie; Åkerman, Gabriella; Lindh, Christian; Teichert, Friederike; Singh, Rajinder; Kippler, Maria; Nermell, Barbro; Raqib, Rubhana; Strömberg, Ulf; Broberg Palmgren, Karin.

I: Free Radical Biology & Medicine, Vol. 48, Nr. 9, 2010, s. 1211-1217.

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Engström, Karin ; Vahter, Marie ; Åkerman, Gabriella ; Lindh, Christian ; Teichert, Friederike ; Singh, Rajinder ; Kippler, Maria ; Nermell, Barbro ; Raqib, Rubhana ; Strömberg, Ulf ; Broberg Palmgren, Karin. / Chronic exposure to cadmium and arsenic strongly influences concentrations of 8-oxo-7,8-dihydro-2'-deoxyguanosine in urine. I: Free Radical Biology & Medicine. 2010 ; Vol. 48, Nr. 9. s. 1211-1217.

RIS

TY - JOUR

T1 - Chronic exposure to cadmium and arsenic strongly influences concentrations of 8-oxo-7,8-dihydro-2'-deoxyguanosine in urine.

AU - Engström, Karin

AU - Vahter, Marie

AU - Åkerman, Gabriella

AU - Lindh, Christian

AU - Teichert, Friederike

AU - Singh, Rajinder

AU - Kippler, Maria

AU - Nermell, Barbro

AU - Raqib, Rubhana

AU - Strömberg, Ulf

AU - Broberg Palmgren, Karin

PY - 2010

Y1 - 2010

N2 - Exposure to arsenic (As), cadmium (Cd) and lead (Pb) may generate oxidative stress, which can be assessed by 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) in urine, a sensitive marker of oxidatively damaged DNA. We have evaluated oxidative stress induced by mixed chronic exposure to As, Cd, Pb, as well as the influence of As metabolism and nutritional status, i.e. ferritin (Ft), selenium (Se), zinc (Zn), manganese (Mn) and body weight. 8-oxodG was measured in urine from 212 women in early pregnancy from Matlab, rural Bangladesh, using LC-MS/MS. Cd and Pb were analyzed in urine and erythrocytes, while Se, Mn and Zn were analyzed in erythrocytes, all by ICPMS. As and As metabolites were analyzed in urine by HPLC-ICPMS. Ferritin was analyzed in plasma by radioimmunoassay. Median concentration of 8-oxodG was 8.3 nmol/L (adjusted for specific gravity), range 1.2-43, corresponding to a median of 4.7 mug/g creatinine, range 1.8-32. 8-oxodG was positively associated with urinary Cd (ss=0.32, p<0.001), urinary As (ss=0.0007, p=0.001), fraction of the monomethylated arsenic metabolite (MMA) in urine (ss=0.0026, p=0.004) and plasma Ft (ss = 0.20, p<0.001). A joint effect was seen for U-Cd and U-As, but whether this effect was additive or multiplicative was difficult to discern.

AB - Exposure to arsenic (As), cadmium (Cd) and lead (Pb) may generate oxidative stress, which can be assessed by 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) in urine, a sensitive marker of oxidatively damaged DNA. We have evaluated oxidative stress induced by mixed chronic exposure to As, Cd, Pb, as well as the influence of As metabolism and nutritional status, i.e. ferritin (Ft), selenium (Se), zinc (Zn), manganese (Mn) and body weight. 8-oxodG was measured in urine from 212 women in early pregnancy from Matlab, rural Bangladesh, using LC-MS/MS. Cd and Pb were analyzed in urine and erythrocytes, while Se, Mn and Zn were analyzed in erythrocytes, all by ICPMS. As and As metabolites were analyzed in urine by HPLC-ICPMS. Ferritin was analyzed in plasma by radioimmunoassay. Median concentration of 8-oxodG was 8.3 nmol/L (adjusted for specific gravity), range 1.2-43, corresponding to a median of 4.7 mug/g creatinine, range 1.8-32. 8-oxodG was positively associated with urinary Cd (ss=0.32, p<0.001), urinary As (ss=0.0007, p=0.001), fraction of the monomethylated arsenic metabolite (MMA) in urine (ss=0.0026, p=0.004) and plasma Ft (ss = 0.20, p<0.001). A joint effect was seen for U-Cd and U-As, but whether this effect was additive or multiplicative was difficult to discern.

KW - Free radicals

KW - Lead

KW - Arsenic

KW - 8-OHdG

KW - Cadmium

KW - Ferritin

KW - Oxidative stress

KW - Pregnancy

U2 - 10.1016/j.freeradbiomed.2010.02.004

DO - 10.1016/j.freeradbiomed.2010.02.004

M3 - Article

VL - 48

SP - 1211

EP - 1217

JO - Free Radical Biology & Medicine

JF - Free Radical Biology & Medicine

SN - 0891-5849

IS - 9

ER -