Circulating nucleosomes as epigenetic biomarkers in pancreatic cancer.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

To improve the prognosis of patients with pancreatic cancer, new biomarkers are required for earlier, pre-symptomatic diagnosis. Epigenetic mutations take place at the earliest stages of tumorigenesis and therefore offer new approaches for detecting and diagnosing disease. Nucleosomes are the repeating subunits of DNA and histone proteins that constitute human chromatin. Because of their release into the circulation, intact nucleosome levels in serum or plasma can serve as diagnostic disease biomarkers, and elevated levels have been reported in various cancers. However, quantifying nucleosomes in the circulation for cancer detection has been challenging due to nonspecific elevation in sera of patients with benign diseases. Here, we report for the first time differential, disease-associated epigenetic profiles of intact cell-free nucleosomes (cfnucleosomes) containing specific DNA and histone modifications as well as histone variants circulating in the blood. The study comprised serum samples from 59 individuals, including 25 patients with resectable pancreatic cancer, 10 patients with benign pancreatic disease, and 24 healthy individuals using Nucleosomics(®), a novel ELISA method.

Detaljer

Författare
Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Kirurgi
Originalspråkengelska
Artikelnummer106
TidskriftClinical Epigenetics
Volym7
StatusPublished - 2015
PublikationskategoriForskning
Peer review utfördJa

Nedladdningar

Ingen tillgänglig data

Relaterad forskningsoutput

Bauden, M., 2017, Lund: Lund University: Faculty of Medicine. 74 s.

Forskningsoutput: AvhandlingDoktorsavhandling (sammanläggning)

Visa alla (1)

Related projects

Roland Andersson, DANIEL ANSARI, Monika Bauden, Carlos Urey Chavez, Johannes Byrling, Linus Aronsson, Katarzyna Said Hilmersson, Bodil Andersson & Agata Sasor

Swedish Government Agency for Innovation Systems (Vinnova)

2007/05/15 → …

Projekt: Forskning

Visa alla (1)