Cisterna Magna Injection in Rats to Study Glymphatic Function

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Cisterna Magna Injection in Rats to Study Glymphatic Function. / Ramos, Marta; Burdon Bechet, Nicholas; Battistella, Roberta; Pavan, Chiara; Xavier, Anna L.R.; Nedergaard, Maiken; Lundgaard, Iben.

I: Methods in molecular biology (Clifton, N.J.), Vol. 1938, 2019, s. 97-104.

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T1 - Cisterna Magna Injection in Rats to Study Glymphatic Function

AU - Ramos, Marta

AU - Burdon Bechet, Nicholas

AU - Battistella, Roberta

AU - Pavan, Chiara

AU - Xavier, Anna L.R.

AU - Nedergaard, Maiken

AU - Lundgaard, Iben

PY - 2019

Y1 - 2019

N2 - The recently discovered glymphatic system, which supports brain-wide clearance of metabolic waste, has become the subject of intense research within the past few years. Its nomenclature arose due to its functionally analogous nature to the lymphatic system in combination with glial cells that are part of its anatomical boundaries. The influx of cerebrospinal fluid (CSF) from perivascular spaces into the brain interstitium acts to clear intraparenchymal solutes. CSF is produced by the choroid plexus and flows from the ventricles to the subarachnoid space via the cisterna magna, and as such the injection of tracer molecules into any one of these spaces could be used for studying CSF movement through the glymphatic system. Of these options, the cisterna magna is most favorable as it offers a route of entry that does not involve craniotomy. Herein we describe the cisterna magna (CM) injection procedure carried out in rats, essential for studying glymphatic influx and efflux dynamics.

AB - The recently discovered glymphatic system, which supports brain-wide clearance of metabolic waste, has become the subject of intense research within the past few years. Its nomenclature arose due to its functionally analogous nature to the lymphatic system in combination with glial cells that are part of its anatomical boundaries. The influx of cerebrospinal fluid (CSF) from perivascular spaces into the brain interstitium acts to clear intraparenchymal solutes. CSF is produced by the choroid plexus and flows from the ventricles to the subarachnoid space via the cisterna magna, and as such the injection of tracer molecules into any one of these spaces could be used for studying CSF movement through the glymphatic system. Of these options, the cisterna magna is most favorable as it offers a route of entry that does not involve craniotomy. Herein we describe the cisterna magna (CM) injection procedure carried out in rats, essential for studying glymphatic influx and efflux dynamics.

KW - Cerebrospinal fluid

KW - Cisterna magna injection

KW - Glymphatic system

KW - Tracer

U2 - 10.1007/978-1-4939-9068-9_7

DO - 10.1007/978-1-4939-9068-9_7

M3 - Article

VL - 1938

SP - 97

EP - 104

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

SN - 1940-6029

ER -