Concentrated protein solutions investigated using acoustic levitation and small-Angle X-ray scattering

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

An acoustically levitated droplet has been used to collect synchrotron SAXS data on human serum albumin protein solutions up to a protein concentration of 400mgml-1. A careful selection of experiments allows for fast data collection of a large amount of data, spanning a protein concentration/solvent concentration space with limited sample consumption (down to 3μL per experiment) and few measurements. The data analysis shows data of high quality that are reproducible and comparable with data from standard flow-Through capillary-based experiments. Furthermore, using this methodology, it is possible to achieve concentrations that would not be accessible by conventional cells. The protein concentration and ionic strength parameter space diagram may be covered easily and the amount of protein sample is significantly reduced (by a factor of 100 in this work). Used in routine measurements, the benefits in terms of protein cost and time spent are very significant.

Detaljer

Författare
Enheter & grupper
Externa organisationer
  • Technical University of Denmark
  • Research Institutes of Sweden (RISE)
  • University of Copenhagen
  • CMC assist ApS
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Fysik

Nyckelord

Originalspråkengelska
Sidor (från-till)396-404
Antal sidor9
TidskriftJournal of Synchrotron Radiation
Volym27
StatusPublished - 2020 mar 1
PublikationskategoriForskning
Peer review utfördJa