Conversion of lignin model compounds by Pseudomonas putida KT2440 and isolates from compost

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Conversion of lignin model compounds by Pseudomonas putida KT2440 and isolates from compost. / Ravi, Krithika; Garcia Hidalgo, Javier; Gorwa-Grauslund, Marie F; Lidén, Gunnar.

I: Applied Microbiology and Biotechnology, Vol. 101, Nr. 12, 06.2017, s. 5059-5070.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

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T1 - Conversion of lignin model compounds by Pseudomonas putida KT2440 and isolates from compost

AU - Ravi, Krithika

AU - Garcia Hidalgo, Javier

AU - Gorwa-Grauslund, Marie F

AU - Lidén, Gunnar

PY - 2017/6

Y1 - 2017/6

N2 - Starting from mature vegetable compost, four bacterial strains were selected using a lignin-rich medium. 16S ribosomal RNA identification of the isolates showed high score similarity with Pseudomonas spp. for three out of four isolates. Further characterization of growth on mixtures of six selected lignin model compounds (vanillin, vanillate, 4-hydroxybenzoate, p-coumarate, benzoate, and ferulate) was carried out with three of the Pseudomonas isolates and in addition with the strain Pseudomonas putida KT2440 from a culture collection. The specific growth rates on benzoate, p-coumarate, and 4-hydroxybenzoate were considerably higher (0.26–0.27 h−1) than those on ferulate and vanillate (0.21 and 0.22 h−1), as were the uptake rates.There was no direct growth of P. putida KT2440 on vanillin, but instead, vanillin was rapidly converted in to vanillate at a rate of 4.87 mmol (gCDW h)−1 after which the accumulated vanillate was taken up. The growth curve reflected a diauxic growthwhen mixtures of the model compounds were used as carbon source. Vanillin, 4-hydroxybenzoate, and benzoate were preferentially consumed first, whereas ferulate was always the last substrate to be taken in. These results contribute to a better understanding of the aromatic metabolism of P. putida in terms of growth and uptake rates, which will be helpful for the utilization of these bacteria as cell factories for upgrading lignin-derived mixtures of aromatic molecules.

AB - Starting from mature vegetable compost, four bacterial strains were selected using a lignin-rich medium. 16S ribosomal RNA identification of the isolates showed high score similarity with Pseudomonas spp. for three out of four isolates. Further characterization of growth on mixtures of six selected lignin model compounds (vanillin, vanillate, 4-hydroxybenzoate, p-coumarate, benzoate, and ferulate) was carried out with three of the Pseudomonas isolates and in addition with the strain Pseudomonas putida KT2440 from a culture collection. The specific growth rates on benzoate, p-coumarate, and 4-hydroxybenzoate were considerably higher (0.26–0.27 h−1) than those on ferulate and vanillate (0.21 and 0.22 h−1), as were the uptake rates.There was no direct growth of P. putida KT2440 on vanillin, but instead, vanillin was rapidly converted in to vanillate at a rate of 4.87 mmol (gCDW h)−1 after which the accumulated vanillate was taken up. The growth curve reflected a diauxic growthwhen mixtures of the model compounds were used as carbon source. Vanillin, 4-hydroxybenzoate, and benzoate were preferentially consumed first, whereas ferulate was always the last substrate to be taken in. These results contribute to a better understanding of the aromatic metabolism of P. putida in terms of growth and uptake rates, which will be helpful for the utilization of these bacteria as cell factories for upgrading lignin-derived mixtures of aromatic molecules.

KW - Compost

KW - Pseudomonas

KW - Lignin

KW - Aromatic compound conversion

KW - Bacterial metabolism

U2 - 10.1007/s00253-017-8211-y

DO - 10.1007/s00253-017-8211-y

M3 - Article

C2 - 28299400

VL - 101

SP - 5059

EP - 5070

JO - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

SN - 1432-0614

IS - 12

ER -