Coomassie Staining as Loading Control in Western Blot Analysis

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

In Western blotting, immunodetection of housekeeping proteins is routinely performed to detect differences in electrophoresis loading. The present work describes a much faster and simpler protein staining method, which is compatible with ordinary blocking conditions. In addition, the method can be used after immunodetection with superior linearity compared to ordinary staining methods. After immunoblotting and staining, protein bands can be further identified using peptide mass fingerprinting.

Detaljer

Författare
Enheter & grupper
Externa organisationer
  • Skåne University Hospital
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Cancer och onkologi

Nyckelord

Originalspråkengelska
Sidor (från-till)1416-1419
TidskriftJournal of Proteome Research
Volym10
Utgivningsnummer3
StatusPublished - 2011
PublikationskategoriForskning
Peer review utfördJa