Critical comparison of performances of superficially porous particles and sub-2 mu m particles under optimized ultra-high pressure conditions

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

The performance of 2.7 mu m superficially porous particles at 600 bar and sub-2 mu m fully porous particles at 1000 bar were compared by the Poppe plot method. Theoretical Poppe plots were first constructed for each stationary phase to compare their kinetic performance at different analysis times. The theory was then verified by experiments under the optimized conditions identified from the Poppe plot calculation. We found that the 2.7 mu m superficially porous particles at 600 bar can provide similar performance compared to the sub-2 mu m fully porous particles at ultra-high pressure (1000bar)when analysis times are very short (e.g. sub-minute). As analysis time increases, the superficially porous particles start to outperform the sub-2 mu m particles and can give much higher efficiencies (e.g. > 2 times higher plate Count) at very long analysis times (>3 h). The comparison was extended to gradient elution of a mixture of pharmaceutical interest by constructing gradient peak capacity Poppe plots and similar behavior was observed. (C) 2009 Elsevier B.V. All rights reserved.

Detaljer

Författare
  • Yuan Zhang
  • Xiaoli Wang
  • Partha Mukherjee
  • Patrik Petersson
Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Cancer och onkologi

Nyckelord

Originalspråkengelska
Sidor (från-till)4597-4605
TidskriftJournal of Chromatography A
Volym1216
Utgivningsnummer21
StatusPublished - 2009
PublikationskategoriForskning
Peer review utfördJa