Crowding-Controlled Cluster Size in Concentrated Aqueous Protein Solutions: Structure, Self- and Collective Diffusion

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift


We investigate the concentration-controlled formation of clusters in β-lactoglobulin (BLG) protein solutions combining structural and dynamical scattering techniques. The static structure factor from small-angle X-ray scattering as well as de-Gennes narrowing in the nanosecond diffusion function D(q) from neutron spin echo spectroscopy support a picture of cluster formation. Using neutron backscattering spectroscopy, a monotonous increase of the average hydrodynamic cluster radius is monitored over a broad protein concentration range, corresponding to oligomeric structures of BLG ranging from the native dimers up to roughly four dimers. The results suggest that BLG forms compact clusters that are static on the observation time scale of several nanoseconds. The presented analysis provides a general framework to access the structure and dynamics of macromolecular assemblies in solution.


  • Michal K. Braun
  • Marco Grimaldo
  • Felix Roosen-Runge
  • Ingo Hoffmann
  • Orsolya Czakkel
  • Michael Sztucki
  • Fajun Zhang
  • Frank Schreiber
  • Tilo Seydel
Enheter & grupper
Externa organisationer
  • University of Tübingen
  • Institut Laue Langevin
  • European Synchrotron Radiation Facility

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Fysikalisk kemi
Sidor (från-till)2590-2596
Antal sidor7
TidskriftJournal of Physical Chemistry Letters
Utgåva nummer12
StatusPublished - 2017 jun 15
Peer review utfördJa