Crystal structures of the Bacillus subtilis prophage lytic cassette proteins XepA and YomS.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

As part of the Virus-X Consortium that aims to identify and characterize novel proteins and enzymes from bacteriophages and archaeal viruses, the genes of the putative lytic proteins XepA from Bacillus subtilis prophage PBSX and YomS from prophage SPβ were cloned and the proteins were subsequently produced and functionally characterized. In order to elucidate the role and the molecular mechanism of XepA and YomS, the crystal structures of these proteins were solved at resolutions of 1.9 and 1.3 Å, respectively. XepA consists of two antiparallel β-sandwich domains connected by a 30-amino-acid linker region. A pentamer of this protein adopts a unique dumbbell-shaped architecture consisting of two discs and a central tunnel. YomS (12.9 kDa per monomer), which is less than half the size of XepA (30.3 kDa), shows homology to the C-terminal part of XepA and exhibits a similar pentameric disc arrangement. Each β-sandwich entity resembles the fold of typical cytoplasmic membrane-binding C2 domains. Only XepA exhibits distinct cytotoxic activity in vivo, suggesting that the N-terminal pentameric domain is essential for this biological activity. The biological and structural data presented here suggest that XepA disrupts the proton motive force of the cytoplasmatic membrane, thus supporting cell lysis.

Detaljer

Författare
  • Stefanie Freitag-Pohl
  • Andrius Jasilionis
  • Maria Håkansson
  • Anders Svensson
  • Rebeka Kovačič
  • Martin Welin
  • Hildegard Watzlawick
  • Lei Wang
  • Josef Altenbuchner
  • Magdalena Plotka
  • Anna Karina Kaczorowska
  • Tadeusz Kaczorowski
  • Eva Nordberg Karlsson
  • Salam Al-Karadaghi
  • Bjorn Walse
  • Arnthor Aevarsson
  • Ehmke Pohl
Enheter & grupper
Externa organisationer
  • Durham University
  • SARomics Biostructures AB
  • Matís ohf
  • University of Gdansk
  • University of Stuttgart
  • Lund University
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Industriell bioteknik
Originalspråkengelska
Sidor (från-till)1028 - 1039
TidskriftActa Crystallographica Section D: Structural Biology
Volym75
StatusPublished - 2019 nov 1
PublikationskategoriForskning
Peer review utfördJa