Crystallization and preliminary X-ray analysis of human dUTPase

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

Human dUTPase, expressed in Escherichia coli, has been crystallized. Single crystals were obtained by the vapour-diffusion technique using 2-propanol and PEG 4000 as precipitants. The enzyme was co-crystallized with the substrate dUTP and a metal chelator EDTA to prevent hydrolysis of the substrate. The crystals belong to the orthorombic space group P212121 with cell dimensions a = 67.51, b = 68.26 and c = 91.00 Å. The crystallographic asymmetric unit contains one trimer of identical subunits.

Detaljer

Författare
  • G Larsson
  • Per-Olof Nyman
  • L A Svensson
Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Strukturbiologi
Originalspråkengelska
Sidor (från-till)1039-1040
TidskriftActa Crystallographica. Section D: Biological Crystallography
VolymD52
Utgåva nummer5
StatusPublished - 1996
PublikationskategoriForskning
Peer review utfördJa