Cultivation technology development of Rhodothermus marinus DSM 16675

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

This work presents an evaluation of batch, fed-batch, and sequential batch cultivation techniques for production of R. marinus DSM 16675 and its exopolysaccharides (EPSs) and carotenoids in a bioreactor, using lysogeny broth (LB) and marine broth (MB), respectively, in both cases supplemented with 10 g/L maltose. Batch cultivation using LB supplemented with maltose (LBmalt) resulted in higher cell density (OD620 = 6.6) than use of MBmalt (OD620 = 1.7). Sequential batch cultivation increased the cell density threefold (OD620 = 20) in LBmalt and eightfold (OD620 = 14) in MBmalt. In both single and sequential batches, the production of carotenoids and EPSs using LBmalt was detected in the exponential phase and stationary phase, respectively, while in MBmalt formation of both products was detectable in both the exponential and stationary phases of the culture. Heteropolymeric EPSs were produced with an overall volumetric productivity (QE) of 0.67 (mg/L h) in MBmalt and the polymer contained xylose. In LB, QE was lower (0.1 mg/L h) and xylose could not be detected in the composition of the produced EPSs. In conclusion, this study showed the importance of a process design and medium source for production of R. marinus DSM 16675 and its metabolites.

Detaljer

Författare
Enheter & grupper
Externa organisationer
  • Lund University
  • Matís ohf
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Industriell bioteknik

Nyckelord

Originalspråkengelska
Sidor (från-till)735-745
Antal sidor11
TidskriftExtremophiles : life under extreme conditions
Volym23
Utgåva nummer6
Tidigt onlinedatum2019 sep 14
StatusPublished - 2019 nov 1
PublikationskategoriForskning
Peer review utfördJa