Culturing and patch clamping of Jurkat T cells and neurons on Al 2 O 3 coated nanowire arrays of altered morphology

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Culturing and patch clamping of Jurkat T cells and neurons on Al 2 O 3 coated nanowire arrays of altered morphology. / Harberts, Jann; Zierold, Robert; Fendler, Cornelius; Koitmäe, Aune; Bayat, Parisa; Fernandez-Cuesta, Irene; Loers, Gabriele; Diercks, Björn Philipp; Fliegert, Ralf; Guse, Andreas H.; Ronning, Carsten; Otnes, Gaute; Borgström, Magnus; Blick, Robert H.

I: RSC Advances, Vol. 9, Nr. 20, 2019, s. 11194-11201.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Harvard

Harberts, J, Zierold, R, Fendler, C, Koitmäe, A, Bayat, P, Fernandez-Cuesta, I, Loers, G, Diercks, BP, Fliegert, R, Guse, AH, Ronning, C, Otnes, G, Borgström, M & Blick, RH 2019, 'Culturing and patch clamping of Jurkat T cells and neurons on Al 2 O 3 coated nanowire arrays of altered morphology', RSC Advances, vol. 9, nr. 20, s. 11194-11201. https://doi.org/10.1039/c8ra05320k

APA

Harberts, J., Zierold, R., Fendler, C., Koitmäe, A., Bayat, P., Fernandez-Cuesta, I., ... Blick, R. H. (2019). Culturing and patch clamping of Jurkat T cells and neurons on Al 2 O 3 coated nanowire arrays of altered morphology. RSC Advances, 9(20), 11194-11201. https://doi.org/10.1039/c8ra05320k

CBE

Harberts J, Zierold R, Fendler C, Koitmäe A, Bayat P, Fernandez-Cuesta I, Loers G, Diercks BP, Fliegert R, Guse AH, Ronning C, Otnes G, Borgström M, Blick RH. 2019. Culturing and patch clamping of Jurkat T cells and neurons on Al 2 O 3 coated nanowire arrays of altered morphology. RSC Advances. 9(20):11194-11201. https://doi.org/10.1039/c8ra05320k

MLA

Vancouver

Harberts J, Zierold R, Fendler C, Koitmäe A, Bayat P, Fernandez-Cuesta I et al. Culturing and patch clamping of Jurkat T cells and neurons on Al 2 O 3 coated nanowire arrays of altered morphology. RSC Advances. 2019;9(20):11194-11201. https://doi.org/10.1039/c8ra05320k

Author

Harberts, Jann ; Zierold, Robert ; Fendler, Cornelius ; Koitmäe, Aune ; Bayat, Parisa ; Fernandez-Cuesta, Irene ; Loers, Gabriele ; Diercks, Björn Philipp ; Fliegert, Ralf ; Guse, Andreas H. ; Ronning, Carsten ; Otnes, Gaute ; Borgström, Magnus ; Blick, Robert H. / Culturing and patch clamping of Jurkat T cells and neurons on Al 2 O 3 coated nanowire arrays of altered morphology. I: RSC Advances. 2019 ; Vol. 9, Nr. 20. s. 11194-11201.

RIS

TY - JOUR

T1 - Culturing and patch clamping of Jurkat T cells and neurons on Al 2 O 3 coated nanowire arrays of altered morphology

AU - Harberts, Jann

AU - Zierold, Robert

AU - Fendler, Cornelius

AU - Koitmäe, Aune

AU - Bayat, Parisa

AU - Fernandez-Cuesta, Irene

AU - Loers, Gabriele

AU - Diercks, Björn Philipp

AU - Fliegert, Ralf

AU - Guse, Andreas H.

AU - Ronning, Carsten

AU - Otnes, Gaute

AU - Borgström, Magnus

AU - Blick, Robert H.

PY - 2019

Y1 - 2019

N2 - Nanowire substrates play an increasingly important role for cell cultures as an approach for hybrid bio-semiconductor junctions. We investigate Jurkat T cells and neurons from mice cultured on Al 2 O 3 coated ordered and randomly distributed nanowires. Cell viability was examined by life/membrane staining reporting comparable viability on planar and nanowire substrates. Imaging the hybrid interface reveals a wrapping of the cell membrane around the very nanowire tip. Patch clamp recordings show similar electrophysiological responses on each type of nanowires compared to planar control substrates. We demonstrate that the morphological characteristic of the nanowire substrate plays a subordinate role which opens up the arena for a large range of nanowire substrates in a functionalized application such as stimulation or sensing.

AB - Nanowire substrates play an increasingly important role for cell cultures as an approach for hybrid bio-semiconductor junctions. We investigate Jurkat T cells and neurons from mice cultured on Al 2 O 3 coated ordered and randomly distributed nanowires. Cell viability was examined by life/membrane staining reporting comparable viability on planar and nanowire substrates. Imaging the hybrid interface reveals a wrapping of the cell membrane around the very nanowire tip. Patch clamp recordings show similar electrophysiological responses on each type of nanowires compared to planar control substrates. We demonstrate that the morphological characteristic of the nanowire substrate plays a subordinate role which opens up the arena for a large range of nanowire substrates in a functionalized application such as stimulation or sensing.

UR - https://doi.org/10.1039/c9ra90030f

U2 - 10.1039/c8ra05320k

DO - 10.1039/c8ra05320k

M3 - Article

VL - 9

SP - 11194

EP - 11201

JO - RSC Advances

T2 - RSC Advances

JF - RSC Advances

SN - 2046-2069

IS - 20

ER -