CXCR4 Signaling Has a CXCL12-Independent Essential Role in Murine MLL-AF9-Driven Acute Myeloid Leukemia

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

Acute myeloid leukemia (AML) is defined by an accumulation of immature myeloid blasts in the bone marrow. To identify key dependencies of AML stem cells in vivo, here we use a CRISPR-Cas9 screen targeting cell surface genes in a syngeneic MLL-AF9 AML mouse model and show that CXCR4 is a top cell surface regulator of AML cell growth and survival. Deletion of Cxcr4 in AML cells eradicates leukemia cells in vivo without impairing their homing to the bone marrow. In contrast, the CXCR4 ligand CXCL12 is dispensable for leukemia development in recipient mice. Moreover, expression of mutated Cxcr4 variants reveals that CXCR4 signaling is essential for leukemia cells. Notably, loss of CXCR4 signaling in leukemia cells leads to oxidative stress and differentiation in vivo. Taken together, our results identify CXCR4 signaling as essential for AML stem cells by protecting them from differentiation independent of CXCL12 stimulation.

Detaljer

Författare
Enheter & grupper
Externa organisationer
  • University of Alabama
  • Skåne University Hospital
  • Brigham and Women's Hospital, Boston
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Cell- och molekylärbiologi
  • Hematologi

Nyckelord

Originalspråkengelska
Artikelnummer107684
TidskriftCell Reports
Volym31
Utgåva nummer8
StatusPublished - 2020 maj 26
PublikationskategoriForskning
Peer review utfördJa

Relaterad forskningsoutput

Ramprasad Ramakrishnan, 2020, Lund: Lund University, Faculty of Medicine. 86 s.

Forskningsoutput: AvhandlingDoktorsavhandling (sammanläggning)

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