Decellularization of the Murine Cardiopulmonary Complex

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

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Decellularization of the Murine Cardiopulmonary Complex. / Mayorca-guiliani, Alejandro E; Rafaeva, Maria; Willacy, Oliver; Madsen, Chris; Reuten, Raphael; Erler, Janine T.

I: Journal of visualized experiments : JoVE, Vol. 171, 30.05.2021.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Harvard

Mayorca-guiliani, AE, Rafaeva, M, Willacy, O, Madsen, C, Reuten, R & Erler, JT 2021, 'Decellularization of the Murine Cardiopulmonary Complex', Journal of visualized experiments : JoVE, vol. 171. https://doi.org/10.3791/61854

APA

Mayorca-guiliani, A. E., Rafaeva, M., Willacy, O., Madsen, C., Reuten, R., & Erler, J. T. (2021). Decellularization of the Murine Cardiopulmonary Complex. Journal of visualized experiments : JoVE, 171. https://doi.org/10.3791/61854

CBE

MLA

Vancouver

Author

Mayorca-guiliani, Alejandro E ; Rafaeva, Maria ; Willacy, Oliver ; Madsen, Chris ; Reuten, Raphael ; Erler, Janine T. / Decellularization of the Murine Cardiopulmonary Complex. I: Journal of visualized experiments : JoVE. 2021 ; Vol. 171.

RIS

TY - JOUR

T1 - Decellularization of the Murine Cardiopulmonary Complex

AU - Mayorca-guiliani, Alejandro E

AU - Rafaeva, Maria

AU - Willacy, Oliver

AU - Madsen, Chris

AU - Reuten, Raphael

AU - Erler, Janine T.

PY - 2021/5/30

Y1 - 2021/5/30

N2 - We present here a decellularization protocol for mouse heart and lungs. It produces structural ECM scaffolds that can be used to analyze ECM topology and composition. It is based on a microsurgical procedure designed to catheterize the trachea and aorta of a euthanized mouse to perfuse the heart and lungs with decellularizing agents. The decellularized cardiopulmonary complex can subsequently be immunostained to reveal the location of structural ECM proteins. The whole procedure can be completed in 4 days.The ECM scaffolds resulting from this protocol are free of dimensional distortions. The absence of cells enables structural examination of ECM structures down to submicron resolution in 3D. This protocol can be applied to healthy and diseased tissue from mice as young as 4-weeks old, including mouse models of fibrosis and cancer, opening the way to determine ECM remodeling associated with cardiopulmonary disease.

AB - We present here a decellularization protocol for mouse heart and lungs. It produces structural ECM scaffolds that can be used to analyze ECM topology and composition. It is based on a microsurgical procedure designed to catheterize the trachea and aorta of a euthanized mouse to perfuse the heart and lungs with decellularizing agents. The decellularized cardiopulmonary complex can subsequently be immunostained to reveal the location of structural ECM proteins. The whole procedure can be completed in 4 days.The ECM scaffolds resulting from this protocol are free of dimensional distortions. The absence of cells enables structural examination of ECM structures down to submicron resolution in 3D. This protocol can be applied to healthy and diseased tissue from mice as young as 4-weeks old, including mouse models of fibrosis and cancer, opening the way to determine ECM remodeling associated with cardiopulmonary disease.

U2 - 10.3791/61854

DO - 10.3791/61854

M3 - Article

C2 - 34125099

VL - 171

JO - Journal of Visualized Experiments

JF - Journal of Visualized Experiments

SN - 1940-087X

ER -