Decreased cortical levels of astrocytic glutamate transport protein GLT-1 in a rat model of posttraumatic epilepsy

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Bibtex

@article{9190a7e348c14226ae1094b0974a1bdc,
title = "Decreased cortical levels of astrocytic glutamate transport protein GLT-1 in a rat model of posttraumatic epilepsy",
abstract = "The extracellular homeostasis of glutamate in the brain is maintained by the efficient uptake into astroglial cells. The high extracellular glutamate levels seen during seizures are therefore probably a result of both an increased synaptic release and a deranged glutamate uptake. In this study we used immuno-blotting technique to measure the cortical levels of the astrocytic glutamate transport protein (GLT-1) and of the glutamate and aspartate transporting protein (GLAST) in an epilepsy model induced by ferrous chloride injection in the cortex of rats. The levels of GLT-1 were lower in epileptic rats than in controls, day 1 and 5 after induction, but not at 3 months. Glial fibrillary protein (GFAP) levels increased with time in the epileptic model, whereas GLAST and beta-tubulin III remained unchanged compared to controls. The results suggest that the transient decrease of GLT-1 could play a role in epileptogenesis, while recurrent seizure activity may be maintained by other mechanisms.",
keywords = "ATP-Binding Cassette Transporters/metabolism, Amino Acid Transport System X-AG, Animals, Astrocytes/drug effects, Cerebral Cortex/metabolism, Disease Models, Animal, Electroencephalography/drug effects, Epilepsy, Post-Traumatic/chemically induced, Ferrous Compounds/adverse effects, Glial Fibrillary Acidic Protein/metabolism, Glutamic Acid/metabolism, Male, Neurons/drug effects, Rats, Rats, Sprague-Dawley, Tubulin/metabolism",
author = "C Samuelsson and E Kumlien and R Flink and D Lindholm and E Ronne-Engstr{\"o}m",
year = "2000",
month = aug,
day = "11",
doi = "10.1016/S0304-3940(00)01284-2",
language = "English",
volume = "289",
pages = "185--8",
journal = "Neuroscience Letters",
issn = "0304-3940",
publisher = "Elsevier",
number = "3",

}