Defining midbrain dopaminergic neuron diversity by single-cell gene expression profiling

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

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Defining midbrain dopaminergic neuron diversity by single-cell gene expression profiling. / Poulin, Jean-Francois; Zou, Jian; Drouin-Ouellet, Janelle; Kim, Kwang-Youn A; Cicchetti, Francesca; Awatramani, Rajeshwar B.

I: Cell Reports, Vol. 9, Nr. 3, 06.11.2014, s. 930-43.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Harvard

Poulin, J-F, Zou, J, Drouin-Ouellet, J, Kim, K-YA, Cicchetti, F & Awatramani, RB 2014, 'Defining midbrain dopaminergic neuron diversity by single-cell gene expression profiling', Cell Reports, vol. 9, nr. 3, s. 930-43. https://doi.org/10.1016/j.celrep.2014.10.008

APA

Poulin, J-F., Zou, J., Drouin-Ouellet, J., Kim, K-Y. A., Cicchetti, F., & Awatramani, R. B. (2014). Defining midbrain dopaminergic neuron diversity by single-cell gene expression profiling. Cell Reports, 9(3), 930-43. https://doi.org/10.1016/j.celrep.2014.10.008

CBE

Poulin J-F, Zou J, Drouin-Ouellet J, Kim K-YA, Cicchetti F, Awatramani RB. 2014. Defining midbrain dopaminergic neuron diversity by single-cell gene expression profiling. Cell Reports. 9(3):930-43. https://doi.org/10.1016/j.celrep.2014.10.008

MLA

Vancouver

Poulin J-F, Zou J, Drouin-Ouellet J, Kim K-YA, Cicchetti F, Awatramani RB. Defining midbrain dopaminergic neuron diversity by single-cell gene expression profiling. Cell Reports. 2014 nov 6;9(3):930-43. https://doi.org/10.1016/j.celrep.2014.10.008

Author

Poulin, Jean-Francois ; Zou, Jian ; Drouin-Ouellet, Janelle ; Kim, Kwang-Youn A ; Cicchetti, Francesca ; Awatramani, Rajeshwar B. / Defining midbrain dopaminergic neuron diversity by single-cell gene expression profiling. I: Cell Reports. 2014 ; Vol. 9, Nr. 3. s. 930-43.

RIS

TY - JOUR

T1 - Defining midbrain dopaminergic neuron diversity by single-cell gene expression profiling

AU - Poulin, Jean-Francois

AU - Zou, Jian

AU - Drouin-Ouellet, Janelle

AU - Kim, Kwang-Youn A

AU - Cicchetti, Francesca

AU - Awatramani, Rajeshwar B

PY - 2014/11/6

Y1 - 2014/11/6

N2 - Effective approaches to neuropsychiatric disorders require detailed understanding of the cellular composition and circuitry of the complex mammalian brain. Here, we present a paradigm for deconstructing the diversity of neurons defined by a specific neurotransmitter using a microfluidic dynamic array to simultaneously evaluate the expression of 96 genes in single neurons. With this approach, we successfully identified multiple molecularly distinct dopamine neuron subtypes and localized them in the adult mouse brain. To validate the anatomical and functional correlates of molecular diversity, we provide evidence that one Vip+ subtype, located in the periaqueductal region, has a discrete projection field within the extended amygdala. Another Aldh1a1+ subtype, located in the substantia nigra, is especially vulnerable in the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) model of Parkinson's disease. Overall, this rapid, cost-effective approach enables the identification and classification of multiple dopamine neuron subtypes, with distinct molecular, anatomical, and functional properties.

AB - Effective approaches to neuropsychiatric disorders require detailed understanding of the cellular composition and circuitry of the complex mammalian brain. Here, we present a paradigm for deconstructing the diversity of neurons defined by a specific neurotransmitter using a microfluidic dynamic array to simultaneously evaluate the expression of 96 genes in single neurons. With this approach, we successfully identified multiple molecularly distinct dopamine neuron subtypes and localized them in the adult mouse brain. To validate the anatomical and functional correlates of molecular diversity, we provide evidence that one Vip+ subtype, located in the periaqueductal region, has a discrete projection field within the extended amygdala. Another Aldh1a1+ subtype, located in the substantia nigra, is especially vulnerable in the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) model of Parkinson's disease. Overall, this rapid, cost-effective approach enables the identification and classification of multiple dopamine neuron subtypes, with distinct molecular, anatomical, and functional properties.

KW - 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine

KW - Aging

KW - Animals

KW - Disease Models, Animal

KW - Dopaminergic Neurons

KW - Gene Expression Profiling

KW - High-Throughput Screening Assays

KW - Mesencephalon

KW - Mice, Inbred C57BL

KW - Parkinson Disease

KW - Journal Article

KW - Research Support, N.I.H., Extramural

KW - Research Support, Non-U.S. Gov't

U2 - 10.1016/j.celrep.2014.10.008

DO - 10.1016/j.celrep.2014.10.008

M3 - Article

VL - 9

SP - 930

EP - 943

JO - Cell Reports

JF - Cell Reports

SN - 2211-1247

IS - 3

ER -