Defining the Minimal Factors Required for Erythropoiesis through Direct Lineage Conversion

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

Erythroid cell commitment and differentiation proceed through activation of a lineage-restricted transcriptional network orchestrated by a group of well characterized genes. However, the minimal set of factors necessary for instructing red blood cell (RBC) development remains undefined. We employed a screen for transcription factors allowing direct lineage reprograming from fibroblasts to induced erythroid progenitors/precursors (iEPs). We show that Gata1, Tal1, Lmo2, and c-Myc (GTLM) can rapidly convert murine and human fibroblasts directly to iEPs. The transcriptional signature of murine iEPs resembled mainly that of primitive erythroid progenitors in the yolk sac, whereas addition of Klf1 or Myb to the GTLM cocktail resulted in iEPs with a more adult-type globin expression pattern. Our results demonstrate that direct lineage conversion is a suitable platform for defining and studying the core factors inducing the different waves of erythroid development.

Detaljer

Författare
Enheter & grupper
Externa organisationer
  • Center of Regenerative Medicine in Barcelona
  • Lund University
  • Rockefeller University
  • University of Melbourne
  • Harvard University
  • Broad Institute
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Cell- och molekylärbiologi
  • Hematologi

Nyckelord

Originalspråkengelska
Sidor (från-till)2550-2562
Antal sidor13
TidskriftCell Reports
Volym15
Utgivningsnummer11
StatusPublished - 2016 jun 14
PublikationskategoriForskning
Peer review utfördJa

Relaterad forskningsoutput

Sandra Capellera Garcia, 2018, Lund: Lund University, Faculty of Medicine. 111 s.

Forskningsoutput: AvhandlingDoktorsavhandling (sammanläggning)

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