Denitrification and DNRA at the Baltic Sea oxic–anoxic interface: Substrate spectrum and kinetics

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Denitrification and DNRA at the Baltic Sea oxic–anoxic interface : Substrate spectrum and kinetics. / Bonaglia, Stefano; Klawonn, Isabell; De Brabandere, Loreto; Deutsch, Barbara; Thamdrup, Bo; Brüchert, Volker.

I: Limnology and Oceanography, Vol. 61, Nr. 5, 01.09.2016, s. 1900-1915.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Harvard

Bonaglia, S, Klawonn, I, De Brabandere, L, Deutsch, B, Thamdrup, B & Brüchert, V 2016, 'Denitrification and DNRA at the Baltic Sea oxic–anoxic interface: Substrate spectrum and kinetics', Limnology and Oceanography, vol. 61, nr. 5, s. 1900-1915. https://doi.org/10.1002/lno.10343

APA

Bonaglia, S., Klawonn, I., De Brabandere, L., Deutsch, B., Thamdrup, B., & Brüchert, V. (2016). Denitrification and DNRA at the Baltic Sea oxic–anoxic interface: Substrate spectrum and kinetics. Limnology and Oceanography, 61(5), 1900-1915. https://doi.org/10.1002/lno.10343

CBE

MLA

Vancouver

Bonaglia S, Klawonn I, De Brabandere L, Deutsch B, Thamdrup B, Brüchert V. Denitrification and DNRA at the Baltic Sea oxic–anoxic interface: Substrate spectrum and kinetics. Limnology and Oceanography. 2016 sep 1;61(5):1900-1915. https://doi.org/10.1002/lno.10343

Author

Bonaglia, Stefano ; Klawonn, Isabell ; De Brabandere, Loreto ; Deutsch, Barbara ; Thamdrup, Bo ; Brüchert, Volker. / Denitrification and DNRA at the Baltic Sea oxic–anoxic interface : Substrate spectrum and kinetics. I: Limnology and Oceanography. 2016 ; Vol. 61, Nr. 5. s. 1900-1915.

RIS

TY - JOUR

T1 - Denitrification and DNRA at the Baltic Sea oxic–anoxic interface

T2 - Limnology and Oceanography

AU - Bonaglia, Stefano

AU - Klawonn, Isabell

AU - De Brabandere, Loreto

AU - Deutsch, Barbara

AU - Thamdrup, Bo

AU - Brüchert, Volker

PY - 2016/9/1

Y1 - 2016/9/1

N2 - The dependence of denitrification and dissimilatory nitrate reduction to ammonium (DNRA) on different electron donors was tested in the nitrate-containing layer immediately below the oxic–anoxic interface (OAI) at three stations in the central anoxic basins of the Baltic Sea. Additionally, pathways and rates of fixed nitrogen transformation were investigated with 15N incubation techniques without addition of donors. Denitrification and anammox were always detected, but denitrification rates were higher than anammox rates. DNRA occurred at two sites and rates were two orders of magnitude lower than denitrification rates. Separate additions of dissolved organic carbon and sulfide stimulated rates without time lag indicating that both organotrophic and lithotrophic bacterial populations were simultaneously active and that they could carry out denitrification or DNRA. Manganese addition stimulated denitrification and DNRA at one station, but it is not clear whether this was due to a direct or indirect effect. Ammonium oxidation to nitrite was detected on one occasion. During denitrification, the production of nitrous oxide (N2O) was as important as dinitrogen (N2) production. A high ratio of N2O to N2 production at one site may be due to copper limitation, which inhibits the last denitrification step. These data demonstrate the coexistence of a range of oxidative and reductive nitrogen cycling processes at the Baltic OAI and suggest that the dominant electron donor supporting denitrification and DNRA is organic matter. Organotrophic denitrification is more important for nitrogen budgets than previously thought, but the large temporal variability in rates calls for long-term seasonal studies.

AB - The dependence of denitrification and dissimilatory nitrate reduction to ammonium (DNRA) on different electron donors was tested in the nitrate-containing layer immediately below the oxic–anoxic interface (OAI) at three stations in the central anoxic basins of the Baltic Sea. Additionally, pathways and rates of fixed nitrogen transformation were investigated with 15N incubation techniques without addition of donors. Denitrification and anammox were always detected, but denitrification rates were higher than anammox rates. DNRA occurred at two sites and rates were two orders of magnitude lower than denitrification rates. Separate additions of dissolved organic carbon and sulfide stimulated rates without time lag indicating that both organotrophic and lithotrophic bacterial populations were simultaneously active and that they could carry out denitrification or DNRA. Manganese addition stimulated denitrification and DNRA at one station, but it is not clear whether this was due to a direct or indirect effect. Ammonium oxidation to nitrite was detected on one occasion. During denitrification, the production of nitrous oxide (N2O) was as important as dinitrogen (N2) production. A high ratio of N2O to N2 production at one site may be due to copper limitation, which inhibits the last denitrification step. These data demonstrate the coexistence of a range of oxidative and reductive nitrogen cycling processes at the Baltic OAI and suggest that the dominant electron donor supporting denitrification and DNRA is organic matter. Organotrophic denitrification is more important for nitrogen budgets than previously thought, but the large temporal variability in rates calls for long-term seasonal studies.

UR - http://www.scopus.com/inward/record.url?scp=84978120575&partnerID=8YFLogxK

U2 - 10.1002/lno.10343

DO - 10.1002/lno.10343

M3 - Article

VL - 61

SP - 1900

EP - 1915

JO - Limnology and Oceanography

JF - Limnology and Oceanography

SN - 1939-5590

IS - 5

ER -