Dependence of macrophage superoxide release on the pulse amplitude of an applied pressure regime: A potential factor at the soft tissue-implant interface

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Failure of soft tissue implants has been largely attributed to the influence of biomaterial surface properties on the foreign body response, but some implant complications, e.g. macrophage accumulation and necrosis, are still not effectively addressed with surface treatments to minimize deleterious biomaterial effects. We explored an alternative explanation for implant failure, linking biocompatibility with implant micromotion-induced pressure fluctuations at the tissue-biomaterial interface. For this purpose, we used a custom in vitro system to characterize the effects of pressure fluctuations on the activity of macrophages, the predominant cells at a healing implant site. Initially, we quantified superoxide production by HL60-derived macrophage-like cells under several different pressure regimes with means of 5-40 mmHg, amplitudes of 0-15 mmHg and frequencies of 0-1.5 Hz. All pressure regimes tested elicited significantly (p2=0.74; p


  • Hainsworth Y. Shin
  • Danielle M. Frechette
  • Nathan Rohner
  • Xiaoyan Zhang
  • David A. Puleo
  • Lars M. Bjursten
Enheter & grupper
Externa organisationer
  • University of Kentucky

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Annan klinisk medicin
  • Annan medicinsk grundvetenskap


Sidor (från-till)E227-E238
TidskriftJournal of Tissue Engineering and Regenerative Medicine
StatusPublished - 2016 mar 1
Peer review utfördJa