Design of recombinant antibody microarrays for cell surface membrane proteomics

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Design of recombinant antibody microarrays for cell surface membrane proteomics. / Dexlin Mellby, Linda; Ingvarsson, Johan; Frendeus, Björn; Borrebaeck, Carl; Wingren, Christer.

I: Journal of Proteome Research, Vol. 7, Nr. 1, 2008, s. 319-327.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

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Dexlin Mellby, Linda ; Ingvarsson, Johan ; Frendeus, Björn ; Borrebaeck, Carl ; Wingren, Christer. / Design of recombinant antibody microarrays for cell surface membrane proteomics. I: Journal of Proteome Research. 2008 ; Vol. 7, Nr. 1. s. 319-327.

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TY - JOUR

T1 - Design of recombinant antibody microarrays for cell surface membrane proteomics

AU - Dexlin Mellby, Linda

AU - Ingvarsson, Johan

AU - Frendeus, Björn

AU - Borrebaeck, Carl

AU - Wingren, Christer

PY - 2008

Y1 - 2008

N2 - Generating proteomic maps of membrane proteins, common targets for therapeutic interventions and disease diagnostics, has turned out to be a major challenge. Antibody-based microarrays are among the novel rapidly evolving proteomic technologies that may enable global proteome analysis to be performed. Here, we have designed the first generation of a scaleable human recombinant scFv antibody microarray technology platform for cell surface membrane proteomics as well as glycomics targeting intact cells. The results showed that rapid and multiplexed profiling of the cell surface proteome (and glycome) could be performed in a highly specific and sensitive manner and that differential expression patterns due to external stimuli could be monitored.

AB - Generating proteomic maps of membrane proteins, common targets for therapeutic interventions and disease diagnostics, has turned out to be a major challenge. Antibody-based microarrays are among the novel rapidly evolving proteomic technologies that may enable global proteome analysis to be performed. Here, we have designed the first generation of a scaleable human recombinant scFv antibody microarray technology platform for cell surface membrane proteomics as well as glycomics targeting intact cells. The results showed that rapid and multiplexed profiling of the cell surface proteome (and glycome) could be performed in a highly specific and sensitive manner and that differential expression patterns due to external stimuli could be monitored.

U2 - 10.1021/pr070257x

DO - 10.1021/pr070257x

M3 - Article

VL - 7

SP - 319

EP - 327

JO - Journal of Proteome Research

T2 - Journal of Proteome Research

JF - Journal of Proteome Research

SN - 1535-3893

IS - 1

ER -