Detailed characterization of plasmids carrying resistance genes using optical DNA mapping

Forskningsoutput: Kapitel i bok/rapport/Conference proceedingKonferenspaper i proceeding

Abstract

We present an assay, based on optical DNA mapping in nanochannels that is capable of characterizing the plasmid content of bacterial isolates resistant to antibiotics in a fast an detailed way. In a single experiment we determine the number of different plasmids in each sample, their size, as well as a barcode that can be used for plasmid identification and tracing. In addition we demonstrate that we can identify resistance genes on individual plasmids using CRISPR/Cas9. We foresee that the assay can be a useful tool all the way from fundamental plasmid biology to diagnostics and surveillance of resistant infections.

Detaljer

Författare
  • Vilhelm Müller
  • Lena K. Nyberg
  • Saair Quaderi
  • Erik Kristiansson
  • Tobias Ambjörnsson
  • Linus Sandegren
  • Fredrik Westerlund
Enheter & grupper
Externa organisationer
  • Chalmers University of Technology
  • Uppsala universitet, Historiska institutionen
  • Lund University
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Medicinsk laboratorie- och mätteknik

Nyckelord

Originalspråkengelska
Titel på värdpublikation20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
FörlagChemical and Biological Microsystems Society
Sidor1561-1562
Antal sidor2
ISBN (elektroniskt)9780979806490
StatusPublished - 2016
PublikationskategoriForskning
Peer review utfördJa
Evenemang20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Irland
Varaktighet: 2016 okt 92016 okt 13

Konferens

Konferens20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
LandIrland
OrtDublin
Period2016/10/092016/10/13