Detection of HLA-D/DR-related DNA polymorphism in HLA-D homozygous typing cells.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

Sequences of different sizes are generated when DNA from homozygous HLA-Dw/DR typing cells are digested with restriction endonuclease and analyzed by hybridization with a HLA-D region class II antigen beta-chain cDNA probe. The patterns of hybridization were highly polymorphic but one endonuclease, BamHI, defined sequences unique to all HLA-Dw/DR specificities 1-8 except HLA-Dw/DR 2 and 6; however, these two specificities were resolved with the enzyme EcoRI. Digestion with other endonucleases such as Pst I results in patterns of restriction fragments that differ between homozygous typing cells of the same HLA-Dw/DR specificity. HLA-D region beta-chain probes permit HLA-D region genotyping at the DNA level and may allow detection of genes controlling the association of HLA specificities with a wide variety of diseases.

Detaljer

Författare
  • D. Owerbach
  • A. Lernmark
  • L. Rask
  • P. A. Peterson
  • P. Platz
  • A. Svejgaard
Externa organisationer
  • Hagedorn Research Institute
Originalspråkengelska
Sidor (från-till)3758-3761
Antal sidor4
TidskriftProceedings of the National Academy of Sciences of the United States of America
Volym80
Utgivningsnummer12
StatusPublished - 1983 jan 1
PublikationskategoriForskning
Peer review utfördJa
Externt publiceradJa