Detection of specific Helicobacter pylori DNA and antigens in stool samples in dyspeptic patients and healthy subjects

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

In this study stool samples from dyspeptic patients and healthy subjects were used for detection of specific Helicobacter pylori antigens and DNA by immunoenzymatic test (PPHpSA) and semi-nested PCR (ureA-PCR), respectively. The H. pylori status was estimated by invasive endoscopy-based rapid urease test and histology or noninvasive urea breath test (UBT), and by serology (ELISA, Western blot). The coincidence of IT pylori-negative invasive tests or UBT and negative antigen or DNA stool tests was very high (mean 95%). The PPHpSA results were found positive for 56% and ureA-PCR for 26% of individuals with H. pylori infection confirmed by invasive tests or UBT. The detection of specific H. pylori antigens and especially DNA in feces is not sufficient as a one-step diagnosis of H. pylori infection.

Detaljer

Författare
  • M Wisniewska
  • Hans-Olof Nilsson
  • L Bak-Romaniszyn
  • T Rechcinski
  • W Bielanski
  • I Planeta-Malecka
  • M Plonka
  • S Konturek
  • Torkel Wadström
  • W Rudnicka
  • M Chmiela
Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Mikrobiologi inom det medicinska området

Nyckelord

Originalspråkengelska
Sidor (från-till)657-665
TidskriftMicrobiology and Immunology
Volym46
Utgåva nummer10
StatusPublished - 2002
PublikationskategoriForskning
Peer review utfördJa