Developing chicken oligodendrocytes express the type IV oligodendrocyte marker T4-O in situ, but not in vitro

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Developing chicken oligodendrocytes express the type IV oligodendrocyte marker T4-O in situ, but not in vitro. / Anderson, Emma S; Bjartmar, Carl; Eriksson Linsmeier, Cecilia; Westermark, Gunilla; Hildebrand, Claes.

I: Neuroscience Letters, Vol. 284, Nr. 1-2, 2000, s. 21-24.

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Anderson, Emma S ; Bjartmar, Carl ; Eriksson Linsmeier, Cecilia ; Westermark, Gunilla ; Hildebrand, Claes. / Developing chicken oligodendrocytes express the type IV oligodendrocyte marker T4-O in situ, but not in vitro. I: Neuroscience Letters. 2000 ; Vol. 284, Nr. 1-2. s. 21-24.

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TY - JOUR

T1 - Developing chicken oligodendrocytes express the type IV oligodendrocyte marker T4-O in situ, but not in vitro

AU - Anderson, Emma S

AU - Bjartmar, Carl

AU - Eriksson Linsmeier, Cecilia

AU - Westermark, Gunilla

AU - Hildebrand, Claes

PY - 2000

Y1 - 2000

N2 - Accumulating data suggest that the oligodendrocyte population includes morphological and biochemical subtypes. We recently reported that a polyclonal antiserum against an unknown antigen, the T4-O molecule, labels a subpopulation of chicken oligodendrocytes, obviously representing the type IV variety of Del Rio Hortega. The present study examines the developmental expression of the T4-O molecule in situ and in vitro. The results show that T4-O immunoreactive cells first appear at E15 in the ventral funiculus. But, oligodendrocytes cultured in vitro with or without neurones do not develop a T4-O immunoreactivity. We conclude that oligodendrocytes in the spinal cord of chicken embryos first express the T4-O molecule some time after onset of myelination, and that the T4-O immunoreactive phenotype does not develop in vitro.

AB - Accumulating data suggest that the oligodendrocyte population includes morphological and biochemical subtypes. We recently reported that a polyclonal antiserum against an unknown antigen, the T4-O molecule, labels a subpopulation of chicken oligodendrocytes, obviously representing the type IV variety of Del Rio Hortega. The present study examines the developmental expression of the T4-O molecule in situ and in vitro. The results show that T4-O immunoreactive cells first appear at E15 in the ventral funiculus. But, oligodendrocytes cultured in vitro with or without neurones do not develop a T4-O immunoreactivity. We conclude that oligodendrocytes in the spinal cord of chicken embryos first express the T4-O molecule some time after onset of myelination, and that the T4-O immunoreactive phenotype does not develop in vitro.

KW - Cell culture

KW - T4-O

KW - Myelination

KW - Development

KW - White matter

KW - Spinal cord

KW - Oligodendrocyte

KW - Chicken

U2 - 10.1016/S0304-3940(00)00989-7

DO - 10.1016/S0304-3940(00)00989-7

M3 - Article

VL - 284

SP - 21

EP - 24

JO - Neuroscience Letters

JF - Neuroscience Letters

SN - 0304-3940

IS - 1-2

ER -