Development of a Sol-gel-assisted Reverse-phase Microarray Platform for Simple and Rapid Detection of Prostate-specific Antigen from Serum

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

A sol-gel-based reverse-phase microarray was developed with improved sensitivity for prostatespecific antigen (PSA) from serum. The pore-sizecontrolled 3D sol-gel matrix was created with a large surface area to capture target molecules densely. Using the optically active sol-gel nanocomposites, human female serum was spiked with PSA and assessed using the reverse-phase protein microarray. The reverse-phase assay exhibited a limit of detection of 1 pg/mL for PSA and a dynamic range of 103 orders of magnitude. Notably, the platform matched the demand of the immunoassay in a simple and feasible manner. Moreover, the platform shortened the total assay time with an increased accuracy of diagnosis.

Detaljer

Författare
Enheter & grupper
Externa organisationer
  • University of Tokyo
  • Dongguk University, Seoul
  • Inje University
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Biomedicinsk laboratorievetenskap/teknologi

Nyckelord

Originalspråkengelska
Sidor (från-till)69-74
Antal sidor6
TidskriftBiochip Journal
Volym12
Utgivningsnummer1
StatusPublished - 2018 mar 1
PublikationskategoriForskning
Peer review utfördJa