Development of large-scale cross-linking mass spectrometry

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Cross-linking mass spectrometry (CLMS) provides distance constraints to study the structure of proteins, multiprotein complexes and protein-protein interactions which are critical for the understanding of protein function. CLMS is an attractive technology to bridge the gap between high-resolution structural biology techniques and proteomic-based interactome studies. However, as outlined in this review there are still several bottlenecks associated with CLMS which limit its application on a proteome-wide level. Specifically, there is an unmet need for comprehensive software that can reliably identify cross-linked peptides from large datasets. In this review we provide supporting information to reason that targeted proteomics of cross-links may provide the required sensitivity to reliably detect and quantify cross-linked peptides and that a reporter ion signature for cross-linked peptides may become a useful approach to increase confidence in the identification process of cross-linked peptides. In addition, the review summarizes the recent advances in CLMS workflows using the analysis of condensin complex in intact chromosomes as a model complex.


Enheter & grupper

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Medicinsk bioteknologi
Sidor (från-till)1055-1066
TidskriftMolecular and Cellular Proteomics
Utgåva nummer6
Tidigt onlinedatum2017 apr 7
StatusPublished - 2018
Peer review utfördJa