Directed antigen targeting in vivo identifies a role for CD103(+) dendritic cells in both tolerogenic and immunogenic T-cell responses.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

The αE integrin chain CD103 identifies a subset of migratory dendritic cells (DCs) in the gut, lung, and skin. To gain further understanding of the function of CD103(+) DCs in regulating adaptive immunity in vivo, we coupled ovalbumin (OVA) to the CD103 antibody M290 (M290.OVA). Intraperitoneal injection of M290.OVA induced OVA-specific CD8(+) and CD4(+) T-cell proliferation in lymph nodes (LNs) of wild-type but not CD103(-/-) mice, or in mice depleted of CD11c(+) cells. In the absence of maturation stimuli, systemic antigen targeting to CD103(+) DCs led to tolerance of CD8(+) T cells, whereas coadministration of adjuvant induced cytotoxic T-lymphocyte (CTL) immunity and antibody production. Mucosal intratracheal application of M290.OVA also induced T-cell proliferation in mediastinal LNs, yet the functional outcome was tolerance that inhibited subsequent development of allergic airway inflammation and immunoglobulin E (IgE) responses to inhaled OVA. These findings identify antigen targeting to CD103(+) DCs as a potential strategy to regulate immune responses in nonlymphoid mucosal tissues.Mucosal Immunology advance online publication 14 December 2011; doi:10.1038/mi.2011.61.

Detaljer

Författare
  • Monika Semmrich
  • M Plantinga
  • Marcus Svensson Frej
  • Heli Uronen-Hansson
  • T Gustafsson
  • Allan Mowat
  • U Yrlid
  • B N Lambrecht
  • William Agace
Enheter & grupper
Externa organisationer
  • Ghent University Hospital
  • Göteborgs universitet
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Immunologi inom det medicinska området
Originalspråkengelska
Sidor (från-till)150-160
TidskriftMucosal Immunology
Volym5
Utgivningsnummer2
StatusPublished - 2012
PublikationskategoriForskning
Peer review utfördJa