Disposable Electrodes for Capacitive Immunosensor
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Disposable electrodes were fabricated by coating chromium (5 nm) and gold (200 nm) on glass strips (5.0 mm x 25.4 mm) and used in a label-free immunosensor. Human Serum albumin (HSA) and its antigen (anti-HSA) were used as a model system. Electropolymerization of o-phenylenediamine was used for the immobilization of anti-HSA by covalent binding. A linear relationship was obtained in the range from 1.0 x 10-(14) to 1.0 x 10-(19) M with a limit of detection of 8.0 x 10(-15) M. Each modified electrode can be reused up to 30 times. The developed system was applied for human serum samples and compared to Albumin BCG method.