Disruption of β2-integrin-cytoskeleton coupling abolishes the signaling capacity of these integrins on granulocytes

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Integrin dependent adhesion and dynamic modulations of the actin network are prerequisites for normal cell locomotion. To investigate whether the actin microfilamentous system does play a role in regulation of β2-integrin-induced signalling, we pretreated granulocytes with staurosporine, a well-known protein kinase inhibitor that has also been shown to disrupt the cytoskeleton of intact cells. Pretreatment with staurosporine completely inhibited the β2-integrin-induced Ca2+ signal and also its ability to trigger actin polymerisation. This inhibition was not related to phosphorylation of the CD18-chain of the β2-integrin, nor to inhibition of protein kinases. Instead, association of β2-integrins with the cortical cytoskeleton, which was observed in untreated cells, was abolished after exposure to staurosporine, indicating that β2-integrin signalling depends on integrin-cytoskeleton interaction. These results suggest not only that the actin network provides an adhesive link to the extracellular matrix and a driving force for the locomotory response, but also that it participates in regulation of β2-integrin signalling during granulocyte locomotion.


Enheter & grupper
Externa organisationer
  • Linköping University
  • Skåne University Hospital

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Cell- och molekylärbiologi
Sidor (från-till)164-169
Antal sidor6
TidskriftBiochemical and Biophysical Research Communications
StatusPublished - 1999
Peer review utfördJa