Distribution of alkaline sphingomyelinase activity in human beings and animals. Tissue and species differences

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Distribution of alkaline sphingomyelinase activity in human beings and animals. Tissue and species differences. / Duan, R D; Hertervig, Erik; Nyberg, L; Hauge, T; Sternby, Berit; Lillienau, Jan; Farooqi, A; Nilsson, Å.

I: Digestive Diseases and Sciences, Vol. 41, Nr. 9, 1996, s. 1801-1806.

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Duan, R D ; Hertervig, Erik ; Nyberg, L ; Hauge, T ; Sternby, Berit ; Lillienau, Jan ; Farooqi, A ; Nilsson, Å. / Distribution of alkaline sphingomyelinase activity in human beings and animals. Tissue and species differences. I: Digestive Diseases and Sciences. 1996 ; Vol. 41, Nr. 9. s. 1801-1806.

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TY - JOUR

T1 - Distribution of alkaline sphingomyelinase activity in human beings and animals. Tissue and species differences

AU - Duan, R D

AU - Hertervig, Erik

AU - Nyberg, L

AU - Hauge, T

AU - Sternby, Berit

AU - Lillienau, Jan

AU - Farooqi, A

AU - Nilsson, Å

PY - 1996

Y1 - 1996

N2 - The alkaline sphingomyelinase (SMase) was first found in rat intestinal brush border. The important roles of this enzyme in digestion of sphingomyelin and in mucosal cell proliferation have been suggested. In the present work, the distribution of the alkaline SMase in the tissues of human beings and animals have been studied. By assaying the enzyme activity in human biopsy samples, we found that the alkaline SMase activity was absent in the stomach, increased in the duodenum, present at high levels in the small intestine, and slightly declined in the colon and rectum. High activities were found similarly in the intestinal contents of the healthy adults and infants. The activities were also found in the intestinal mucosa of rats, normal and germ-free mice, and hamsters with the same distribution pattern as in humans, but not in the intestinal mucosa of guinea pigs. Apart from the intestinal tract, a SMase activity preferring alkaline pH was identified in human and guinea pig bile, but not in the bile of rat, pig, sheep, and cow. No activity was found in either pancreatic tissue or pancreatic juice in all species tested, and none was detected in human urine and milk. In conclusion, alkaline SMase exists predominantly in the digestive system with considerable tissue and species differences.

AB - The alkaline sphingomyelinase (SMase) was first found in rat intestinal brush border. The important roles of this enzyme in digestion of sphingomyelin and in mucosal cell proliferation have been suggested. In the present work, the distribution of the alkaline SMase in the tissues of human beings and animals have been studied. By assaying the enzyme activity in human biopsy samples, we found that the alkaline SMase activity was absent in the stomach, increased in the duodenum, present at high levels in the small intestine, and slightly declined in the colon and rectum. High activities were found similarly in the intestinal contents of the healthy adults and infants. The activities were also found in the intestinal mucosa of rats, normal and germ-free mice, and hamsters with the same distribution pattern as in humans, but not in the intestinal mucosa of guinea pigs. Apart from the intestinal tract, a SMase activity preferring alkaline pH was identified in human and guinea pig bile, but not in the bile of rat, pig, sheep, and cow. No activity was found in either pancreatic tissue or pancreatic juice in all species tested, and none was detected in human urine and milk. In conclusion, alkaline SMase exists predominantly in the digestive system with considerable tissue and species differences.

KW - alkaline sphingomyelinase

KW - intestine

KW - bile

KW - human beings

KW - species differences

U2 - 10.1007/BF02088748

DO - 10.1007/BF02088748

M3 - Article

VL - 41

SP - 1801

EP - 1806

JO - Digestive Diseases and Sciences

T2 - Digestive Diseases and Sciences

JF - Digestive Diseases and Sciences

SN - 0163-2116

IS - 9

ER -