Divergent T follicular helper cell requirement for IgA and IgE production to peanut during allergic sensitization

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Divergent T follicular helper cell requirement for IgA and IgE production to peanut during allergic sensitization. / Zhang, Biyan; Liu, Elise; Gertie, Jake A; Joseph, Julie; Xu, Lan; Pinker, Elisha Y; Waizman, Daniel A; Catanzaro, Jason; Hamza, Kedir Hussen; Lahl, Katharina; Gowthaman, Uthaman; Eisenbarth, Stephanie C.

I: Science Immunology, Vol. 5, Nr. 47, eaay2754, 08.05.2020.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Harvard

Zhang, B, Liu, E, Gertie, JA, Joseph, J, Xu, L, Pinker, EY, Waizman, DA, Catanzaro, J, Hamza, KH, Lahl, K, Gowthaman, U & Eisenbarth, SC 2020, 'Divergent T follicular helper cell requirement for IgA and IgE production to peanut during allergic sensitization', Science Immunology, vol. 5, nr. 47, eaay2754. https://doi.org/10.1126/sciimmunol.aay2754

APA

Zhang, B., Liu, E., Gertie, J. A., Joseph, J., Xu, L., Pinker, E. Y., ... Eisenbarth, S. C. (2020). Divergent T follicular helper cell requirement for IgA and IgE production to peanut during allergic sensitization. Science Immunology, 5(47), [eaay2754]. https://doi.org/10.1126/sciimmunol.aay2754

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Author

Zhang, Biyan ; Liu, Elise ; Gertie, Jake A ; Joseph, Julie ; Xu, Lan ; Pinker, Elisha Y ; Waizman, Daniel A ; Catanzaro, Jason ; Hamza, Kedir Hussen ; Lahl, Katharina ; Gowthaman, Uthaman ; Eisenbarth, Stephanie C. / Divergent T follicular helper cell requirement for IgA and IgE production to peanut during allergic sensitization. I: Science Immunology. 2020 ; Vol. 5, Nr. 47.

RIS

TY - JOUR

T1 - Divergent T follicular helper cell requirement for IgA and IgE production to peanut during allergic sensitization

AU - Zhang, Biyan

AU - Liu, Elise

AU - Gertie, Jake A

AU - Joseph, Julie

AU - Xu, Lan

AU - Pinker, Elisha Y

AU - Waizman, Daniel A

AU - Catanzaro, Jason

AU - Hamza, Kedir Hussen

AU - Lahl, Katharina

AU - Gowthaman, Uthaman

AU - Eisenbarth, Stephanie C

N1 - Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.

PY - 2020/5/8

Y1 - 2020/5/8

N2 - Immunoglobulin A (IgA) is the dominant antibody isotype in the gut and has been shown to regulate microbiota. Mucosal IgA is also widely believed to prevent food allergens from penetrating the gut lining. Even though recent work has elucidated how bacteria-reactive IgA is induced, little is known about how IgA to food antigens is regulated. Although IgA is presumed to be induced in a healthy gut at steady state via dietary exposure, our data do not support this premise. We found that daily food exposure only induced low-level, cross-reactive IgA in a minority of mice. In contrast, induction of significant levels of peanut-specific IgA strictly required a mucosal adjuvant. Although induction of peanut-specific IgA required T cells and CD40L, it was T follicular helper (TFH) cell, germinal center, and T follicular regulatory (TFR) cell-independent. In contrast, IgG1 and IgE production to peanut required TFH cells. These data suggest an alternative paradigm in which the cellular mechanism of IgA production to food antigens is distinct from IgE and IgG1. We developed an equivalent assay to study this process in stool samples from healthy, nonallergic humans, which revealed substantial levels of peanut-specific IgA that were stable over time. Similar to mice, patients with loss of CD40L function had impaired titers of gut peanut-specific IgA. This work challenges two widely believed but untested paradigms about antibody production to dietary antigens: (i) the steady state/tolerogenic response to food antigens includes IgA production and (ii) TFH cells drive food-specific gut IgA.

AB - Immunoglobulin A (IgA) is the dominant antibody isotype in the gut and has been shown to regulate microbiota. Mucosal IgA is also widely believed to prevent food allergens from penetrating the gut lining. Even though recent work has elucidated how bacteria-reactive IgA is induced, little is known about how IgA to food antigens is regulated. Although IgA is presumed to be induced in a healthy gut at steady state via dietary exposure, our data do not support this premise. We found that daily food exposure only induced low-level, cross-reactive IgA in a minority of mice. In contrast, induction of significant levels of peanut-specific IgA strictly required a mucosal adjuvant. Although induction of peanut-specific IgA required T cells and CD40L, it was T follicular helper (TFH) cell, germinal center, and T follicular regulatory (TFR) cell-independent. In contrast, IgG1 and IgE production to peanut required TFH cells. These data suggest an alternative paradigm in which the cellular mechanism of IgA production to food antigens is distinct from IgE and IgG1. We developed an equivalent assay to study this process in stool samples from healthy, nonallergic humans, which revealed substantial levels of peanut-specific IgA that were stable over time. Similar to mice, patients with loss of CD40L function had impaired titers of gut peanut-specific IgA. This work challenges two widely believed but untested paradigms about antibody production to dietary antigens: (i) the steady state/tolerogenic response to food antigens includes IgA production and (ii) TFH cells drive food-specific gut IgA.

U2 - 10.1126/sciimmunol.aay2754

DO - 10.1126/sciimmunol.aay2754

M3 - Article

VL - 5

JO - Science Immunology

JF - Science Immunology

SN - 2470-9468

IS - 47

M1 - eaay2754

ER -