DNA barcoding of fish larvae reveals uncharacterised biodiversity in tropical peat swamps of New Guinea, Indonesia

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Abstract

The Indonesian archipelago, Borneo, Sumatra and West New Guinea (Papua), hosts half of the world's known tropical peat swamps, which support a significant proportion of the estimated biodiversity on Earth. However, several species groups that inhabit peat swamp environments remain poorly characterised and their biology, particularly during early life stages, is not well understood. In the present study we characterised larval and juvenile fish biodiversity, as well as spatial and temporal variability, in a pristine peat swamp environment of the River Kumbe in West New Guinea, Indonesia, based on analysis of the mitochondrial cytochrome-c oxidase subunit 1 (COI) sequence (501bp). Altogether, 10 fish species were detected in the peat swamp habitat during the larval and juvenile stages, whereas 13 additional species were caught at older stages. Twelve species were detected only in a single site, whereas some species, such as the Western archerfish (Toxotes oligolepis) and Lorentz's grunter (Pingalla lorentzi), were observed in all sampling sites. The occurrence of fish larvae also varied temporally for several species. In contrast with many earlier DNA barcoding studies in fish, we were not able to determine the species identity for a large proportion of sequenced larvae (68%) because of the lack of corresponding COI sequences in the reference dataset. Unidentified sequences clustered into five separate monophyletic clades. Based on genetic divergences, the putative taxonomic origin for the five morphotypes are Atherinidae, Osteoglossidae, Terapontidae and Gobiidae.

Detaljer

Författare
Enheter & grupper
Externa organisationer
  • University of Turku
  • Ministry of Marine Affairs and Fisheries, Indonesia
  • Estonian University of Life Sciences - EMÜ
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Zoologi
  • Ekologi
Originalspråkengelska
Sidor (från-till)1079-1087
Antal sidor9
TidskriftMarine and Freshwater Research
Volym68
Utgivningsnummer6
StatusPublished - 2017
PublikationskategoriForskning
Peer review utfördJa