DNA sample cleanup using deterministic lateral displacement

Forskningsoutput: Kapitel i bok/rapport/Conference proceedingKonferenspaper i proceeding

Abstract

Optical mapping relies on the preparation of fluorescent DNA. DNA must be imaged with good signal to noise and therefore the background of unwanted DNA fragments, fluorescent dyes and other reagents need to be removed. We use deterministic lateral displacement to separate 48.5 kbp DNA from < 10 kbp DNA. We also show the removal of 48.5 kbp DNA fragments from a background of fluorescent ATTO647N molecules and the recovery of >50 kbp molecules from a background of shorter digested fragments. In both cases improving signal to noise during imaging.

Detaljer

Författare
Enheter & grupper
Externa organisationer
  • Tel-Aviv University
  • University of Birmingham
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Medicinsk laboratorie- och mätteknik

Nyckelord

Originalspråkengelska
Titel på värdpublikation20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
FörlagChemical and Biological Microsystems Society
Sidor1527-1528
Antal sidor2
ISBN (elektroniskt)9780979806490
StatusPublished - 2016
PublikationskategoriForskning
Peer review utfördJa
Evenemang20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Irland
Varaktighet: 2016 okt 92016 okt 13

Konferens

Konferens20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
LandIrland
OrtDublin
Period2016/10/092016/10/13