Dose-dependent stimulation of bone induction by basic fibroblast growth factor in rats

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Dose-dependent stimulation of bone induction by basic fibroblast growth factor in rats. / Aspenberg, Per; Thorngren, Karl Göran; Lohmander, L. Stefan.

I: Acta Orthopaedica, Vol. 62, Nr. 5, 1991, s. 481-484.

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TY - JOUR

T1 - Dose-dependent stimulation of bone induction by basic fibroblast growth factor in rats

AU - Aspenberg, Per

AU - Thorngren, Karl Göran

AU - Lohmander, L. Stefan

PY - 1991

Y1 - 1991

N2 - Implantation of demineralized bone matrix in rodents elicits a series of cellular events leading to the formation of new bone inside and adjacent to the implant. This process is believed to be initiated by an inductive protein present in bone matrix, and local growth factors may further regulate the process. We have previously shown that local application of recombinant human basic fibroblast growth factor (bFGF) in a carboxymethyl cellulose gel to demineralized bone matrix implants increases the bone yield as measured by calcium content 3 weeks after implantation in rats. We now report that this increase was seen at 3 and 4 weeks, but not earlier or later. Further, the stimulatory effect was seen with doses from 3 to 75 ng per implant. A dose of 0.6 or 380 ng did not increase the bone yield, and 1,900 ng had a marked inhibitory effect. This narrow dosage optimum may reflect the complex actions of the growth factor.

AB - Implantation of demineralized bone matrix in rodents elicits a series of cellular events leading to the formation of new bone inside and adjacent to the implant. This process is believed to be initiated by an inductive protein present in bone matrix, and local growth factors may further regulate the process. We have previously shown that local application of recombinant human basic fibroblast growth factor (bFGF) in a carboxymethyl cellulose gel to demineralized bone matrix implants increases the bone yield as measured by calcium content 3 weeks after implantation in rats. We now report that this increase was seen at 3 and 4 weeks, but not earlier or later. Further, the stimulatory effect was seen with doses from 3 to 75 ng per implant. A dose of 0.6 or 380 ng did not increase the bone yield, and 1,900 ng had a marked inhibitory effect. This narrow dosage optimum may reflect the complex actions of the growth factor.

UR - http://www.scopus.com/inward/record.url?scp=0026014659&partnerID=8YFLogxK

U2 - 10.3109/17453679108996650

DO - 10.3109/17453679108996650

M3 - Article

VL - 62

SP - 481

EP - 484

JO - Acta Orthopaedica

T2 - Acta Orthopaedica

JF - Acta Orthopaedica

SN - 1745-3682

IS - 5

ER -