Drosophila melanogaster deoxyribonucleoside kinase activates gemcitabine

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Drosophila melanogaster deoxyribonucleoside kinase activates gemcitabine. / Knecht, Wolfgang; Mikkelsen, Nils Egil; Clausen, Anders Ranegaard; Willer, Mette; Eklund, Hans; Gojkovic, Zoran; Piskur, Jure.

I: Biochemical and Biophysical Research Communications, Vol. 382, Nr. 2, 2009, s. 430-433.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Harvard

Knecht, W, Mikkelsen, NE, Clausen, AR, Willer, M, Eklund, H, Gojkovic, Z & Piskur, J 2009, 'Drosophila melanogaster deoxyribonucleoside kinase activates gemcitabine', Biochemical and Biophysical Research Communications, vol. 382, nr. 2, s. 430-433. https://doi.org/10.1016/j.bbrc.2009.03.041

APA

Knecht, W., Mikkelsen, N. E., Clausen, A. R., Willer, M., Eklund, H., Gojkovic, Z., & Piskur, J. (2009). Drosophila melanogaster deoxyribonucleoside kinase activates gemcitabine. Biochemical and Biophysical Research Communications, 382(2), 430-433. https://doi.org/10.1016/j.bbrc.2009.03.041

CBE

MLA

Vancouver

Author

Knecht, Wolfgang ; Mikkelsen, Nils Egil ; Clausen, Anders Ranegaard ; Willer, Mette ; Eklund, Hans ; Gojkovic, Zoran ; Piskur, Jure. / Drosophila melanogaster deoxyribonucleoside kinase activates gemcitabine. I: Biochemical and Biophysical Research Communications. 2009 ; Vol. 382, Nr. 2. s. 430-433.

RIS

TY - JOUR

T1 - Drosophila melanogaster deoxyribonucleoside kinase activates gemcitabine

AU - Knecht, Wolfgang

AU - Mikkelsen, Nils Egil

AU - Clausen, Anders Ranegaard

AU - Willer, Mette

AU - Eklund, Hans

AU - Gojkovic, Zoran

AU - Piskur, Jure

PY - 2009

Y1 - 2009

N2 - Drosophila melanogaster multisubstrate deoxyribonucleoside kinase (Dm-dNK) can additionally sensitize human cancer cell lines towards the anti-cancer drug gemcitabine. We show that this property is based on the Dm-dNK ability to efficiently phosphorylate gemcitabine. The 2.2 angstrom resolution structure of DmdNK in complex with gemcitabine shows that the residues Tyr70 and Arg105 play a crucial role in the firm positioning of gemcitabine by extra interactions made by the fluoride atoms. This explains why gemcitabine is a good substrate for Dm-dNK(. (C) 2009 Elsevier Inc. All rights reserved.

AB - Drosophila melanogaster multisubstrate deoxyribonucleoside kinase (Dm-dNK) can additionally sensitize human cancer cell lines towards the anti-cancer drug gemcitabine. We show that this property is based on the Dm-dNK ability to efficiently phosphorylate gemcitabine. The 2.2 angstrom resolution structure of DmdNK in complex with gemcitabine shows that the residues Tyr70 and Arg105 play a crucial role in the firm positioning of gemcitabine by extra interactions made by the fluoride atoms. This explains why gemcitabine is a good substrate for Dm-dNK(. (C) 2009 Elsevier Inc. All rights reserved.

KW - Structure-function relationship

KW - Salvage pathway

KW - Cancer

KW - Gene-therapy

KW - Nucleoside analogs

KW - Deoxyribonucleoside kinase

U2 - 10.1016/j.bbrc.2009.03.041

DO - 10.1016/j.bbrc.2009.03.041

M3 - Article

VL - 382

SP - 430

EP - 433

JO - Biochemical and Biophysical Research Communications

T2 - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 1090-2104

IS - 2

ER -