Early B-cell factor (o/e-1) is a promoter of adipogenesis and involved in control of genes important for terminal adipocyte differentiation.

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Early B-cell factor (o/e-1) is a promoter of adipogenesis and involved in control of genes important for terminal adipocyte differentiation. / Åkerblad, Peter; Lind, Ulrika; Liberg, David; Bamberg, Krister; Sigvardsson, Mikael.

I: Molecular and Cellular Biology, Vol. 22, Nr. 22, 2002, s. 8015-8025.

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T1 - Early B-cell factor (o/e-1) is a promoter of adipogenesis and involved in control of genes important for terminal adipocyte differentiation.

AU - Åkerblad, Peter

AU - Lind, Ulrika

AU - Liberg, David

AU - Bamberg, Krister

AU - Sigvardsson, Mikael

PY - 2002

Y1 - 2002

N2 - Olf-1/early B-cell factor (O/E-1) is a transcription factor important for B-lymphocyte and neuronal gene regulation. Here we report that all three known O/E genes (O/E-1, -2, and -3) are expressed in mouse adipose tissue and are upregulated during adipocyte differentiation. Forced expression of O/E-1 in either the preadipocyte cell line 3T3-L1 or mouse embryonic fibroblasts augmented adipogenesis, and constitutive expression of O/E-1 in uncommitted NIH 3T3 fibroblasts led to initiation of adipocyte differentiation. Furthermore, a dominant negative form of O/E-1 partially suppressed 3T3-L1 adipogenesis, indicating that expression from endogenous O/E target genes is required for 3T3-L1 terminal differentiation. Thus, our data point to the importance of O/E target genes for adipocyte differentiation and suggest a novel role for O/E-1 as an initiator and stimulator of adipogenesis.

AB - Olf-1/early B-cell factor (O/E-1) is a transcription factor important for B-lymphocyte and neuronal gene regulation. Here we report that all three known O/E genes (O/E-1, -2, and -3) are expressed in mouse adipose tissue and are upregulated during adipocyte differentiation. Forced expression of O/E-1 in either the preadipocyte cell line 3T3-L1 or mouse embryonic fibroblasts augmented adipogenesis, and constitutive expression of O/E-1 in uncommitted NIH 3T3 fibroblasts led to initiation of adipocyte differentiation. Furthermore, a dominant negative form of O/E-1 partially suppressed 3T3-L1 adipogenesis, indicating that expression from endogenous O/E target genes is required for 3T3-L1 terminal differentiation. Thus, our data point to the importance of O/E target genes for adipocyte differentiation and suggest a novel role for O/E-1 as an initiator and stimulator of adipogenesis.

U2 - 10.1128/MCB.22.22.8015-8025.2002

DO - 10.1128/MCB.22.22.8015-8025.2002

M3 - Article

VL - 22

SP - 8015

EP - 8025

JO - Molecular and Cellular Biology

JF - Molecular and Cellular Biology

SN - 0270-7306

IS - 22

ER -